| 地高辛标记探针原位杂交法检测人脑胶质瘤组织猴病毒40的表达 |
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| 引用本文： | 陈东,步星耀,高冬玲,姜国忠,张云汉. 地高辛标记探针原位杂交法检测人脑胶质瘤组织猴病毒40的表达[J]. 郑州大学学报(医学版), 2001, 36(2): 163-165 |
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| 作者姓名： | 陈东 步星耀 高冬玲 姜国忠 张云汉 |
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| 作者单位： | 1. 河南医科大学第一附属医院病理科
2. 河南省人民医院神经外科 |
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| 基金项目： | 河南省杰出青年基金资助项目 |
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| 摘 要： | 目的 :检测猴病毒 40 (SV40 )DNA在人脑胶质瘤中的定位表达 ,探讨其在脑胶质瘤发生发展中的生物学意义。方法 :采用地高辛标记探针原位杂交技术 ,检测 2 5 6例人脑胶质瘤和 11例正常脑组织标本。结果 :SV40DNA定位于胶质瘤细胞核 ,阳性细胞呈弥漫性或局灶性分布 ;SV40DNA阳性率 40 .6 % (10 4/2 5 6 ) ,正常脑组织均未检测出SV40DNA ;SV40DNA阳性率与病理分级无关 (P >0 .0 5 )。结论 :SV40感染与人脑胶质瘤病因学密切相关 ;地高辛标记探针原位杂交技术 ,是探讨SV40病毒核酸在组织中定位、分布和感染机制的一种新的特异性方法。
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| 关 键 词： | 胶质瘤 猴病毒40 DNA |
| 修稿时间： | 2000-06-25 |
Expression of simian virus 40 DNA in human brain glioma tissues |
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| CHEN Dong,BU Xingyao,GAO Dongling,Jiang Guozhong,Zhang Yunhan. Expression of simian virus 40 DNA in human brain glioma tissues[J]. Journal of Zhengzhou University: Med Sci, 2001, 36(2): 163-165 |
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| Authors: | CHEN Dong BU Xingyao GAO Dongling Jiang Guozhong Zhang Yunhan |
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| Abstract: | Aim: To detect the location of simian virus 40 (SV40) DNA and its probable biological function in oncogenesis of human brain gliomas.Methods:A total of 256 cases of human brain glioma tissues and 11 cases of normal brain tissues were studied for SV40 DNA by digoxin labeled probe in situ hybridization.Results:Hybridization signals were detected within the nuclei of glioma cells and the positive cells distributed diffusely or focally.SV40 DNA positivity rate was 40.6% in brain glioma tissues,whereas none of the normal tissues had positive results.The correlation brain between SV40 DNA positivity rate and pathological degree of tumor was not apparent(P>0.05).Conclusion:SV40 infection is closely related to etiology of glioma.The technique of digoxigenin labeled probe in situ hybridization is a new specific method in studying the location,distribution and infection mechanisms of SV40 DNA. |
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| Keywords: | glioma simian virus 40 DNA |
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