| 高效液相色谱法制备连翘酯苷对照品 |
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| 引用本文： | 吴昭晖,谭晓梅,罗佳波,贺丰. 高效液相色谱法制备连翘酯苷对照品[J]. 南方医科大学学报, 2005, 25(11): 1398-1399 |
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| 作者姓名： | 吴昭晖 谭晓梅 罗佳波 贺丰 |
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| 作者单位： | 1. 南方医科大学分析测试中心, 广东, 广州, 510515;2. 南方医科大学中医系, 广东, 广州, 510515 |
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| 摘 要： | 目的 研究从中药材连翘中分离制备连翘酯苷对照品的高效液相色谱方法。方法 连翘药材经水提醇沉淀后,经聚酰胺柱层析得粗提物,粗提物用RP-HPLC法分离制备连翘酯苷单体。结果 分离制备的连翘酯苷纯度可达98%。结论 该方法上样量大,操作简单,提取物纯度高,可用于连翘酯苷对照品的大量制备。
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| 关 键 词： | 高效液相色谱法 连翘酯苷/制备 连翘 |
| 文章编号： | 1000-2588(2005)11-1398-02 |
| 修稿时间： | 2005-07-13 |
Preparation of forsythiaside from Forsythia suspensa by high-performance liquid chromatography |
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| WU Zhao-hai,TAN Xiao-mei,LUO Jia-bo,HE Feng. Preparation of forsythiaside from Forsythia suspensa by high-performance liquid chromatography[J]. Journal of Southern Medical University, 2005, 25(11): 1398-1399 |
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| Authors: | WU Zhao-hai TAN Xiao-mei LUO Jia-bo HE Feng |
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| Abstract: | Objective To prepare forsythiaside from Forsythia suspensa by high-performance liquid chromatography (HPLC). Method A crude extract of Forsythia suspensa was prepared by water extraction and alcohol precipitation prior to polyamide column chromatography. Forsythiaside was prepared from the crude extract by RP-HPLC. Results Analysis with HPLC showed that the purity of the prepared forsythiaside reached 98%. Conclusion HPLC is simple and effective for the preparation of forsythiaside from Forsythia suspensa |
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| Keywords: | high-performance liquid chromatography forsythiaside/ preparation Forsythia suspensa |
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