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| 壬基苯酚对豚鼠心室肌细胞L-型钙电流的影响 | |
| 引用本文： | 高青华,朱彤,郭凤,杨磊,赵美眯,李刚,郝丽英.壬基苯酚对豚鼠心室肌细胞L-型钙电流的影响[J].中国医科大学学报,2010,39(2):81-83. |
| 作者姓名： | 高青华 朱彤 郭凤 杨磊 赵美眯 李刚 郝丽英 |
| 作者单位： | 1. 中国医科大学,药学院药物毒理学教研室,沈阳,110001;中国医科大学,基础医学院生理学教研室,沈阳,110001 2. 东北大学,环境生物研究室,沈阳,110004 3. 中国医科大学,药学院药物毒理学教研室,沈阳,110001 |
| 基金项目： | 国家自然科学基金资助项目(30670761;30671726;30870907) |
| 摘 要： | 目的观察环境激素壬基苯酚(NP)对豚鼠心室肌细胞L-型钙电流(ICa-L)的影响。方法应用酶机械法分离豚鼠单个心室肌细胞;应用膜片钳全细胞方法观察NP对单个心室肌细胞ICa-L的影响。结果NP可抑制不同膜电位下的ICa-L,NP(10-6mol·L-1、10-5mol·L-1)使ICa-L峰值电流密度从(-3.2±1.5)pA.pF-1减少到(-1.6±0.8)pA·pF-1(P<0.01)和(-1.4±0.7)pA·pF-1(P<0.01);但对ICa-L激活动力学曲线无明显影响。结论NP可剂量依赖性抑制豚鼠心室肌细胞ICa-L。 |
| 关 键 词： | 壬基苯酚 L-型钙电流 膜片钳 |
Effects of Nonylphenol on L-type Ca~(2+) Currents in Isolated Guinea-pig Ventricular Myocytes |
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| GAO Qing-hua , ZHU Tong , GUO Feng , YANG Lei , ZHAO Mei-mi , LI Gang , HAO Li-ying.Effects of Nonylphenol on L-type Ca~(2+) Currents in Isolated Guinea-pig Ventricular Myocytes[J].Journal of China Medical University,2010,39(2):81-83. | |
| Authors: | GAO Qing-hua ZHU Tong GUO Feng YANG Lei ZHAO Mei-mi LI Gang HAO Li-ying |
| Institution: | GAO Qing-hua1,2,ZHU Tong3,GUO Feng1,YANG Lei3,ZHAO Mei-mi1,LI Gang3,HAO Li-ying1(1.Department of Pharmaceutical Toxicology,College of pharmacy,China Medical University,Shenyang 110001,China,2.Department of Physiology,College of Basic Medical Sciences,3.Lab of Environmental Biology , Engineering,Northeastern University,Shenyang 110004,China) |
| Abstract: | Objective To observe the effects of nonylphenol(NP)on L-type Ca2+ currents(ICa-L)in isolated guinea-pig ventricular myocytes.Methods The ventricular myocytes were isolated from guinea-pig hearts.The L-type Ca2+ currents in the ventricular myocytes treated with NP were measured with whole cell patch-clamp technique.Results Nonylphenol inhibited ICa-L at different potentials.Nonylphenol(10-6 mol·L-1,10-5 mol·L-1)reduced the peak amplitude of ICa-L from-3.2±1.5 pA·pF-1 to-1.6±0.8 pA·pF-1(P < 0.01)and-1.4±0.7 pA·pF-1(P < 0.01),respectively.Nonylphenol did not influence the activation curve of ICa-L significantly.Conclusion Nonylphenol could inhibit the L-type calcium currents in isolated guinea-pig ventricular myocytes with a concentration-dependent manner. |
| Keywords: | nonylphenol L-type Ca2+ currents patch clamp technique |
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