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| CagA＋及VacA＋幽门螺杆菌对克拉霉素的耐药性突变分析 | |
| 引用本文： | 黄衍强,欧平,周喜汉,黄赞松,李晓华,岑朝. CagA＋及VacA＋幽门螺杆菌对克拉霉素的耐药性突变分析[J]. 山东医药, 2010, 50(20): 37-39 |
| 作者姓名： | 黄衍强 欧平 周喜汉 黄赞松 李晓华 岑朝 |
| 作者单位： | 1. 右江民族医学院,广西百色,533000 2. 贺州学院 3. 右江民族医学院附属医院 |
| 基金项目： | 广西自然科学基金项目，广西教育厅课题 |
| 摘 要： | 目的分析CagA＋及VacA＋的幽门螺杆菌（Hp）对克拉霉素耐药与23S rRNA基因点突变的关系。方法采集右江民族医学院附属医院2006~2008年确诊为Hp感染患者的胃窦部黏膜样本进行Hp分离培养和鉴定,PCR扩增CagA＋及VacA＋基因,E-test进行药敏实验,PCR方法扩增23S rRNA基因,基因测序检测克拉霉素耐药菌株的点突变。结果对克拉霉素耐药的CagA＋及VacA＋Hp菌株均存在23S rRNA基因v功能区第2144位和第2143位A-G突变,而敏感菌株没有发现该位点突变。结论Hp对克拉霉素耐药的23S rRNA基因A2143G、A2144G点发生突变,与基因分型无关。 |
| 关 键 词： | 幽门螺杆菌 耐药性 克拉霉素 CagA＋ VacA＋ |
Genetic mutation analysis of CagA~+ and VacA~+ Helicobacter pylori resistance to clarithromycin |
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| HUANG Yan-qiang,OU Ping,ZHOU Xi-han,HUANG Zan-song,LI Xiao-hua,CEN Chao. Genetic mutation analysis of CagA~+ and VacA~+ Helicobacter pylori resistance to clarithromycin[J]. Shandong Medical Journal, 2010, 50(20): 37-39 | |
| Authors: | HUANG Yan-qiang OU Ping ZHOU Xi-han HUANG Zan-song LI Xiao-hua CEN Chao |
| Affiliation: | HUANG Yan-qiang1,OU Ping,ZHOU Xi-han,HUANG Zan-song,LI Xiao-hua,CEN Chao(1 Youjiang Medical College for Nationalities,Baise 533000,P.R.China) |
| Abstract: | Objective To analyze the relationship between CagA+ and VacA+ Helicobacter pylori(Hp) resistance to clarithromycin and the 23S rRNA genetic mutation.Methods Hp strains were isolated from gastric mucosa biopsies of patients with peptic ulcer or gastritis in youjiang medical college for nationalities from 2006 to 2008.CagA+,VacA+ gene fragment were amplified by PCR.Clarithromycin resistance was determined by using E-test.Mutations in clarithromycin resistant strains were identified by measure of gene sequence... |
| Keywords: | CagA+ VacA+ |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |