枸杞黄酮对H2O2损伤的血管内皮细胞NO及NOS作用的研究

目的：研究枸杞黄酮对过氧化氢(H 2 O2)损伤的血管内皮细胞一氧化氮(NO)及一氧化氮合酶(NOS)作用。方法以人脐静脉内皮细胞(HUVEC)为研究对象,建立 H 2 O2诱导 HUVEC 的氧化损伤模型。实验分为正常对照组、H 2 O2组、维生素C(VitC)组、枸杞黄酮低、中、高浓度组(枸杞黄酮100 mg/L、200 mg/L 和400 mg/L 预处理)。用 MTT 法观察枸杞黄酮对 H 2 O2损伤的血管内皮细胞活性的影响,观察枸杞黄酮类化合物对氧化应激损伤细胞过氧化物丙二醛(MDA)、人总一氧化氮合成酶(TNOS)、诱导型一氧化氮合成酶(iNOS)、及 NO 的作用。结果(1)MTT 结果表明 H 2 O2损伤后内皮细胞生长抑制率(IR)为38.8%；枸杞黄酮低、中、高浓度组 IR 分别为34.0%、30.7%、25.5%,与 H 2 O2组比较差异有统计学意义(P <0.01)；(2)与正常对照组相比较,H 2 O2组细胞 NO 活性降低,而 TNOS、iNOS、MDA 生成增加。与 H 2 O2组相比较,枸杞黄酮低、中、高浓度组中NO、TNOS、iNOS、MDA 的变化则相反,与枸杞黄酮呈剂量依赖性(P <0.01)。结论枸杞黄酮对 H 2 O2所致人血管内皮细胞损伤有保护作用,且保护效果与枸杞黄酮呈一定相关性。

The effects Medlar flavone on NO and NOS in H2 O2 damaged vascular endothelial cells

Objective To study of effects Medlar flavone on NO and NOS in H 2 O2 damaged vascular endothelial cells.Meth-ods Human Umbilical Vein Endothelial Cells(HUVEC)have been set as research object.HUVEC cells was damaged by H 2 O2 in-duced oxidative stress.Experiments were divided into normal control group,H 2 O2 injury group,VitC control group,and low,medi-um,high flavonoids protective groups(100,200,400 mg/L).MTT method was used to observe cells activity.The effects Medlar fla-vone on MDA、TNOS、iNOS and NO in H 2 O2 damaged vascular endothelial cells was observed.Results (1)MTT result demonstra-ted the IR of HUVEC cells was 38.8% in H 2 O2 injury group.Which in low,medium,high flavonoids protective groups were 34.0%,30.7%,25.5%.All were lower than H 2 O2 injury group(P <0.01).(2)Compared with normal control group,NOS activity was decreased in H 2 O2 injury cell,while TNOS,iNOS,NO was more.Compared H 2 O2 group,the change of NO,TNOS,iNOS, MDA was opposite in flavone intervention group,and which were dose dependent (P <0.01 ).Conclusion Medlar flavone has a protective effect on H 2 O2 induced HUVEC cells injury,and there may be some kind of relevance between them.