Role of reactive oxygen in phospholipase A2 activation by ischemia/reperfusion of the rat kidney |
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Authors: | Shinichi Goto Harumasa Nakamura Hiroaki Morooka Yoshiaki Terao Osamu Shibata Koji Sumikawa |
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Affiliation: | (1) Department of Anesthesiology, Nagasaki University School of Medicine, Nagasaki City 852-8501, Japan, JP |
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Abstract: | Purpose. To investigate the role of phospholipase A2 (PLA2) in reperfusion injury of the kidney in an in vivo animal model, renal mitochondrial PLA2 activity was measured under three different conditions. Methods. Male Wistar rats (n = 72) anesthetized with pentobarbital underwent renal ischemia surgically for 45 min and were reperfused for the indicated time (renal ischemia/reperfusion). Treatments included reperfusion for various predetermined periods (phase 1), exposure to hyperbaric oxygen (phase 2), and administration of reactive oxygen species (ROS) scavenger (phase 3). Thereafter, each kidney was harvested, and mitochondrial PLA2 activity was measured by a radioisotope technique. Results. Ischemia/reperfusion resulted in time-related PLA2 activation in the renal mitochondria up to 48 h of reperfusion after renal ischemia. Renal mitochondrial PLA2 activity was further augmented by hyperbaric oxygen exposure prior to reperfusion, whereas administration of the ROS scavengers suppressed mitochondrial PLA2 activity. Conclusion. These data suggest that ROS may play an important role in the in vivo activation of PLA2 associated with renal ischemia/reperfusion. Received for publication on July 6, 1998; accepted on November 30, 1998 |
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Keywords: | : Phospholipase A2 Rat kidney Ischemia/reperfusion Superoxide dismutase Dimethylthiourea |
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