| 脑脊液体外培养骨髓间充质干细胞 |
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| 引用本文： | 沈忆新,王鹏,石恩东.脑脊液体外培养骨髓间充质干细胞[J].中国组织工程研究与临床康复,2011,15(36). |
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| 作者姓名： | 沈忆新 王鹏 石恩东 |
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| 作者单位： | 1. 苏州大学附属第二医院骨科,江苏省苏州市,215004
2. 山东省交通医院,山东省济南市,250031 |
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| 摘 要： | 背景:细胞移植是脊髓损伤的一种有效治疗手段,但目前尚缺乏脑脊液对细胞影响的实验依据.目的:动态观察体外脑脊液培养骨髓间充质干细胞的变化.方法:采用密度梯度离心结合贴壁培养法分离纯化大鼠骨髓间充质干细胞,并传代扩增.选择生长良好的第3,4代细胞接种于脑脊液中,通过细胞免疫化学染色法鉴定脑脊液对细胞表型的影响.取生长良好的第5代细胞,分别用含小牛血清的L-DMEM和脑脊液培养.结果与结论:运用密度梯度离心结合贴壁培养法能成功有效地分离纯化大鼠骨髓间充质干细胞,表型鉴定结果为CD90、CD106、CD71、CD29阳性,CD45阴性.脑脊液培养后细胞仍为骨髓间充质干细胞形态,并可见少量小圆细胞,表现为神经样细胞,表形鉴定结果为CD90、CD106、CD71、CD29阳性,CD45阴性,神经元特异性烯醇化酶呈弱阳性(<1%).细胞具有相似的S形生长曲线,生长曲线基本相似.提示骨髓间充质干细胞在脑脊液培养基中可继续生长和增殖,无诱导分化作用,且细胞对生长环境有高度的适应性.
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| 关 键 词： | 骨髓间充质干细胞 脑脊液 分离培养 表型 细胞鉴定 |
Influence of cerebrospinal fluid on in vitro culture of bone marrow mesenchymal stem cells |
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| Shen Yi-xin,Wang Peng,Shi En-dong.Influence of cerebrospinal fluid on in vitro culture of bone marrow mesenchymal stem cells[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(36). |
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| Authors: | Shen Yi-xin Wang Peng Shi En-dong |
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| Institution: | Shen Yi-xin1,Wang Peng2,Shi En-dong2 1Department of Orthopedics,Second Affiliated Hospital of Soochow University,Suzhou 215004,Liaoning Province,China,2Shandong Jiaotong University,Jinan 250031,Shandong Province |
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| Abstract: | BACKGROUND:Cell transplantation is a promising strategy for treatment of spinal cord injury.But the current experimental evidence about cerebrospinal fluid(CSF) effect on the cells is lack.OBJECTIVE:To study the CSF effects on the in vitro culture of bone marrow mesenchymal stem cells(BMSCs).METHODS:BMSCs were isolated and purified by density gradient centrifugation and adherent culture,and BMSCs were passaged in vitro.The third and fourth passage cells were cultured in CSD to observe cells growth condition... |
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