复发性自然流产患者外周血自然杀伤细胞免疫表型检测

目的 检测复发性自然流产(RSA)患者外周血自然杀伤(NK)细胞的免疫表型。方法 应用流式细胞术检测27例RSA妊娠患者、41例RSA非妊娠患者、32例正常妊娠者和25例正常非妊娠者外周血CD56dimCD 16+、CD56brightCD16+/-、CD69+、HLA-DR+ NK细胞表达并进行比较。结果RSA妊娠组、RSA非妊娠组、正常妊娠组和正常非妊娠组外周血CD56dimCD16+ NK细胞占总NK细胞的比例分别为(88.69.±5.86)％、(79.25±9.31)％、(79.24±10.09)％、(75.49±11.96)％;CD56brightCD16+/- NK细胞的比例分别为(8.18±5.54)％、( 12.20±6.49)％、( 13.13±8.65)％、( 11.53±6.23)％；CD69+ NK细胞的比例分别为(3.42±2.13)％、(2.36±1.72)％、(2.68±1.81)％、(2.08±1.73)％；HLA-DR+ NK细胞的比例分别为(15.26±9.32)％、(9.96±7.18)％、( 10.57±8.05)％、(9.64±6.12)％。与RSA非妊娠组、正常妊娠组及正常非妊娠组比较，RSA妊娠组CD56dimCD16+、CD69+、HLA-DR+ NK细胞显著升高（均P＜0.05）。与RSA非妊娠组及正常妊娠组比较，RSA妊娠组CD56brightCD16+/- NK细胞明显降低（均P＜0.05）。结论 RSA妊娠患者外周血中NK细胞CD56dimCD16+、CD69+和HLA-DR+表达增高，而CD56brightCD16+/-表达下降，可能在RSA的发生发展中起着一定的作用。

Immunophenotypes detection of peripheral nature killer cells in patients with recurrent spontaneous abortion

Objective To determine the immunophenotypes of peripheral nature killer (NK) cells in patients with recurrent spontaneous abortion (RSA). Methods Flow cytometry was used to determine the expressions of CD56dimCD16+, CD56brightD16+/-, CD69+ and HLA-DR+ NK cells in peripheral blood samples from 27 pregnant and 41 non-pregnant RSA patients, 32 normal pregnant and 25 non-pregnant women, and the expressions were compared among 4 groups. Results The percentage of total peripheral NK cells from pregnant and non-pregnant RSA women, normal pregnant and non-pregnant women was (88.69± 5.86)％, (79.25±9.31 )％, (79.24±10.09)％ and (75.49±11.96)％, respectively, for CD56dimCD16+ NK cells; (8.18 ± 5.54)％ , (12.20 ± 6.49)％ , (13.13 ± 8.65)％ and (11.53 ± 6.23)％ , respectively, for CD56brightCD16+/- NK cells; (3.42±2.13)％ , (2.36± 1.72)％, (2.68± 1.81)％ and (2.08± 1.73)％ ,respectively, for CD69+ NK cells; (15.26±9.32)％, (9.96±7.18)％, (10.57±8.05)％ and (9.64±6.12)％,respectively, for HLA-DR+ NK cells. Compared with non-pregnant RSA women, normal pregnant or non-pregnant women, pregnant RSA women showed significantly higher percentage of CD56dimCD16% CD69+ and HLA-DR+ NK cells (all P＜0.05). Compared with non-pregnant RSA and normal pregnant women, pregnant RSA women showed significantly lower percentage of CD56brightCD16+/- NK cells (both P＜0.05).Conclusions The increased expression of CD56dimCD16+, CD69+ and HLA-DR+ NK cells and decreased expression of CD56brightCD16+/- NK cells in peripheral blood of the RSA pregnant patients may play a role in the development and progression of RSA.