| 羌活鱼含药血清对大鼠骨髓间充质干细胞增殖及成骨分化的影响 |
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| 引用本文： | 谢兴文,许伟,李宁. 羌活鱼含药血清对大鼠骨髓间充质干细胞增殖及成骨分化的影响[J]. 中国组织工程研究与临床康复, 2011, 15(32): 6016-6020. DOI: 10.3969/j.issn.1673-8225.2011.32.029 |
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| 作者姓名： | 谢兴文 许伟 李宁 |
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| 作者单位： | 1. 甘肃省中医院,甘肃省兰州市,730050
2. 甘肃中医学院,甘肃省兰州市,730000 |
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| 摘 要： | 背景:羌活鱼具有促进骨折愈合的作用,但其具体的作用机制和其有效成分的研究目前尚不清楚。目的:观察不同浓度羌活鱼含药血清对体外培养大鼠骨髓间充质干细胞成骨分化的影响。方法:以羌活鱼研粉剂灌服大鼠制得羌活鱼含药血清,灌服等体积生理盐水制得对照血清;采用贴壁筛选法培养大鼠骨髓间充质干细胞,并以不同浓度(2.5%,5%,7.5%,10%)羌活鱼含药血清干预第3代大鼠骨髓间充质干细胞,通过MTT法检测细胞增殖情况;分别测定细胞碱性磷酸酶活性、钙盐沉积量、骨钙素、Ι型胶原表达量以及钙化结节数。结果与结论:5%和7.5%浓度含药血清可促进细胞增殖、成骨性分化,尤以5%含药血清最为明显,其碱性磷酸酶活性、钙盐沉积量、Ι型胶原含量、骨钙素含量和钙化结节数均显著高于空白对照组(P<0.01)。羌活鱼经口服后的代谢产物可刺激大鼠骨髓间充质干细胞增殖,并能促进其成骨性分化,推测其可能是羌活鱼续断接骨的作用机制之一。
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| 关 键 词： | 羌活鱼 血清 骨髓间充质干细胞 增殖 成骨分化 |
Effects of serum containing Bing's giant salamander on the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells |
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| Xie Xin-wen,Xu Wei,Li Ning. Effects of serum containing Bing's giant salamander on the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(32): 6016-6020. DOI: 10.3969/j.issn.1673-8225.2011.32.029 |
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| Authors: | Xie Xin-wen Xu Wei Li Ning |
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| Affiliation: | Xie Xin-wen1,Xu Wei2,Li Ning2 1Chinese Medicine Hospital of Gansu Province,Lanzhou 730050,Gansu Province,China,2Gansu College of Traditional Chinese Medicine,Lanzhou 730000 |
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| Abstract: | BACKGROUND: Bing's giant salamander has the effect to enhance the fracture healing, but the action mechanism and active constituent are still unclear. OBJECTIVE: To investigate the effects of the serum of Bing's giant salamander on the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs) by different concentrations. METHODS: Rats were given Bing's giant salamander by high, moderate and low concentration, respectively, and their serum was obtained after 7 days. The ... |
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