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| 体外高浓度葡萄糖培养及黄芪干预人外周血内皮祖细胞的数量和增殖与分化 | |
| 引用本文： | 徐寒松,雷闽湘,孔德明,向慧,谢晓云.体外高浓度葡萄糖培养及黄芪干预人外周血内皮祖细胞的数量和增殖与分化[J].中国组织工程研究与临床康复,2011,15(32):6027-6031. |
| 作者姓名： | 徐寒松 雷闽湘 孔德明 向慧 谢晓云 |
| 作者单位： | 1. 贵阳中医学院第二附属医院内分泌科,贵州省贵阳市,550003 2. 中南大学湘雅医院内分泌科,湖南省长沙市,410008 3. 贵州省人民医院心理科,贵州省贵阳市,550003 4. 中南大学湘雅三医院内分泌科,湖南省长沙市,410008 |
| 基金项目： | 国家自然科学基金项目，贵州省优秀科技教育人才省长基金 |
| 摘 要： | 背景:内皮祖细胞数量及功能受损是糖尿病血管并发症发生发展的重要环节,黄芪对多种原因引起内皮功能障碍具有保护作用,可以有效保护血管内皮功能。目的:观察高浓度葡萄糖及黄芪干预对人外周血内皮祖细胞数量、增殖及分化影响。方法:不同浓度葡萄糖孵育内皮祖细胞24h以及30mmol/L葡萄糖孵育内皮祖细胞不同时间;内皮祖细胞经20g/L黄芪预处理24h后,再加入30mmol/L葡萄糖培养24h。结果与结论:高葡萄糖以浓度及时间依赖方式减少内皮祖细胞的数量,降低内皮祖细胞的增殖及向内皮细胞系分化能力(P0.05或0.01),给予黄芪预处理后,内皮祖细胞数量明显增加,增殖及向内皮细胞系分化能力明显增强(P<0.05或0.01)。提示高葡萄糖以浓度及时间依赖方式减少外周血内皮祖细胞数量,降低内皮祖细胞的增殖及向内皮细胞系分化能力,黄芪可以改善高葡萄糖对内皮祖细胞的损伤作用。 |
| 关 键 词： | 内皮祖细胞 黄芪 葡萄糖 增殖 分化 |
Effects of high glucose and Astragalus Membranaceus on the number, proliferation, and differentiation of endothelial progenitor cells from human peripheral blood in vitro |
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| Xu Han-song,Lei Min-xiang,Kong De-ming,Xiang Hui,Xie Xiao-yun.Effects of high glucose and Astragalus Membranaceus on the number, proliferation, and differentiation of endothelial progenitor cells from human peripheral blood in vitro[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(32):6027-6031. | |
| Authors: | Xu Han-song Lei Min-xiang Kong De-ming Xiang Hui Xie Xiao-yun |
| Institution: | Xu Han-song1,Lei Min-xiang2,Kong De-ming1,Xiang Hui3,Xie Xiao-yun4 1Department of Endocrinology,the Second Affiliated Hospital of Guiyang College of TCM,Guiyang 550003,Guizhou Province,China,2Department of Endocrinology,Xiangya Hospital of Central South University,Changsha 410008,Hunan Province,3Department of Psychology,Guizhou Provincial People's Hospital,4Department of Endocrinology,Third Xiangya Hospital of Central South University,Changsha... |
| Abstract: | BACKGROUND: The quantity and impaired function of endothelial progenitor cells (EPCs) is the important link of diabetic vascular complications. Astragalus Membranaceus (AMI) can protect endothelium of dysfunction which caused by various reasons and can effectively protect the function of vascular endothelium. OBJECTIVE: To investigate the effects of high glucose and AMI on the number, proliferation, and differentiation of EPCs from human peripheral blood in vitro. METHODS: EPCs were incubated with various c... |
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