| 混合骨髓移植后过继免疫治疗小鼠白血病 |
| |
| 引用本文： | 王存邦,白海,葸瑞,张茜,周进茂,赵强,潘耀柱. 混合骨髓移植后过继免疫治疗小鼠白血病[J]. 中国组织工程研究与临床康复, 2011, 15(36). DOI: 10.3969/j.issn.1673-8225.2011.36.025 |
| |
| 作者姓名： | 王存邦 白海 葸瑞 张茜 周进茂 赵强 潘耀柱 |
| |
| 作者单位： | 解放军兰州军区兰州总医院全军血液病中心,甘肃省兰州市,730050 |
| |
| 基金项目： | 全军"十一五"杰出人才基金资助项目，兰州军区医药科研基金项目 |
| |
| 摘 要： | 背景:急性白血病自体造血干细胞移植后复发率高,异基因造血干细胞移植后移植相关病死率高,混合造血干细胞移植及移植后过继免疫治疗有可能取长补短,提高疗效.目的:观察自体骨髓混合H-2半相合异体骨髓移植后供体淋巴细胞输注+白细胞介素2治疗对小鼠白血病的疗效.方法:将Balb/c小鼠经直线加速器照射3 Gy后分为白血病模型组、白血病模型照射组、混合移植组、自体骨髓移植组,均尾静脉注射5×105 K562(GFP+/NeoR+)或K562(GFP-/NeoR-)细胞.7 d后6 Gy照射,自体骨髓移植组移植自体骨髓细胞或联合白细胞介素2治疗;混合移植组移植小鼠自体骨髓细胞混合1/10的H-2半相合异体骨髓细胞后应用白细胞介素2或联合供体淋巴细胞输注治疗.4周后行小鼠外周血及骨髓细胞形态检查,外周血细胞亚群、GFP及NeoR基因测定,肝、脾匀浆细胞GFP和NeoR基因测定.结果与结论:白血病模型组小鼠因骨髓造血功能衰竭于20 d内全部死亡,白血病模型照射组小鼠因造血功能衰竭于14 d内全部死亡;自体骨髓移植组、混合移植组均有多少不等小鼠无白血病存活超过28 d,且混合骨髓移植后及自体骨髓移植后应用白细胞介素2治疗可提高白血病小鼠长期无病生存率,在此基础上联合供体淋巴细胞输注可更进一步提高白血病小鼠长期无病生存率.
|
| 关 键 词： | 混合骨髓移植 供者淋巴细胞输注 白细胞介素2 小鼠白血病模型 过继免疫治疗 |
Adoptive immunotherapy for leukemia in mice after autologous bone marrow mixed with H-2 haploidentical allogeneic bone marrow transplantation |
| |
| Wang Cun-bang,Bai Hai,Xi Rui,Zhang Qian,Zhou Jin-mao,Zhao Qiang,Pan Yao-zhu. Adoptive immunotherapy for leukemia in mice after autologous bone marrow mixed with H-2 haploidentical allogeneic bone marrow transplantation[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(36). DOI: 10.3969/j.issn.1673-8225.2011.36.025 |
| |
| Authors: | Wang Cun-bang Bai Hai Xi Rui Zhang Qian Zhou Jin-mao Zhao Qiang Pan Yao-zhu |
| |
| Affiliation: | Wang Cun-bang,Bai Hai,Xi Rui,Zhang Qian,Zhou Jin-mao,Zhao Qiang,Pan Yao-zhu Hematology Center,General Hospital of Lanzhou Military Area Command of Chinese PLA,Lanzhou 730050,Gansu Province,China |
| |
| Abstract: | BACKGROUND:Auto hemopoietic stem cell transplantation(Auto-HSCT) has a high relapse rate in acute leukemia;allo-HSCT has a high incidence of transplant-related mortality.It may increase curative effect when leukemia patients are administered adoptive immunotherapy post mixed-HSCT.OBJECTIVE:To explore the curative effect of using donor lymphocyte infusion combined with interleukin-2(DLI+IL-2) after autologous bone marrow mixed with H-2 haploidentical allogeneic bone marrow transplantation(MBMT) in mice with ... |
| |
| Keywords: | |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
