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| CXCL12/CXCR4生物学轴对骨髓间充质干细胞修复脊髓损伤的影响 | |
| 引用本文： | 范东艳,刘然,徐福春,王苹.CXCL12/CXCR4生物学轴对骨髓间充质干细胞修复脊髓损伤的影响[J].中国组织工程研究与临床康复,2011,15(36):6651-6656. |
| 作者姓名： | 范东艳 刘然 徐福春 王苹 |
| 作者单位： | 1. 西藏大学医学院,西藏自治区拉萨市,850000 2. 长春市中心血站,吉林省长春市,130033 3. 吉林大学第一医院耳鼻喉-头颈外科,吉林省长春市,130021 |
| 摘 要： | 背景:研究发现不同途径移植骨髓间充质干细胞均能向脊髓损伤部位迁移,进而发挥治疗作用。目的:探讨CXCL12/CXCR4生物学轴对骨髓间充质干细胞趋向脊髓损伤部位迁移的作用。方法:采用改进的脊椎骨破坏法制备脊髓损伤模型。假手术组只打开皮肤,不损伤脊髓且不作任何干预;模型组于造模后第2天采用腰骶鞘内注射5μL生理盐水;细胞移植组于造模后第2天采用腰骶鞘内移植5μL骨髓间充质干细胞。结果与结论:①荧光显微镜下可见,横切的脊髓损伤局部有大量的标记细胞聚集,而在损伤部位远端1cm处,仅见少量的标记骨髓间充质干细胞。②骨髓间充质干细胞表达中等水平的趋化因子CXCL12,其特异性结合受体CXCR4也有低水平表达。③脊髓损伤7d后,局部CXCL12表达增强,主要集中在脊髓损伤部位的皮质区域,而在损伤部位1cm以外的脊髓组织未见大量表达的CXCL12。CXCR4蛋白表达没有明显的时间效应。④检测CXCL12mRNA的转录水平发现细胞移植组的CXCR4转录水平明显高于假手术组和模型组,损伤后14d脊髓损伤局部CXCL12的转录水平最强,21d时降低,CXCL12的局部转录水平明显高于远端。⑤脊髓损伤部位也表达趋化因子CXCR4,但其表达水平没有时程差异。损伤局部的CXCR4转录水平略高于远端,但差异无显著性意义。说明CXCL12/CXCR4生物学轴参与骨髓间充质干细胞向脊髓损伤区域迁移。 |
| 关 键 词： | 脊髓损伤 骨髓间充质干细胞 CXCL12 CXCR4 迁移 |
CXCL12/CXCR4 biology axis effects on the repair of spinal cord injury with bone marrow mesenchymal stem cells |
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| Fan Dong-yan,Liu Yan,Xu Fu-chun,Wang Ping.CXCL12/CXCR4 biology axis effects on the repair of spinal cord injury with bone marrow mesenchymal stem cells[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(36):6651-6656. | |
| Authors: | Fan Dong-yan Liu Yan Xu Fu-chun Wang Ping |
| Institution: | Fan Dong-yan1,Liu Yan2,Xu Fu-chun1,Wang Ping3 1Tibet University Medical School,Lhasa 850000,Tibet Autonomous Region,China,2Center Blood Bank of Changchun,Changchun 130033,Jilin Province,3Department of Otolaryngology-Head and Neck Surgery,First Hospital of Jilin University,Changchun 130021 |
| Abstract: | BACKGROUND:The research discovered that bone marrow mesenchymal stem cells(BMSCs) through different approaches can migrate to the injured site of the spinal cord,and play curative effects.OBJECTIVE:To discuss the role of CXCL12/CXCR4 biology axis on the migration of BMSCs into the injured site after spinal cord injury(SCI).METHODS:SCI models were prepared using modified spinal damage method.There was no intervention in the sham operation group beside skin open.In the model group,lumbar intrathecal injection... |
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