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| 5-氮杂胞苷诱导人脐带间充质干细胞向心肌样细胞的分化 | |
| 引用本文： | 阮中宝,杨向军,陈各才,朱莉,李伟,杨兵,欧阳溪,潘少辉.5-氮杂胞苷诱导人脐带间充质干细胞向心肌样细胞的分化[J].中国组织工程研究与临床康复,2011,15(36):6705-6708. |
| 作者姓名： | 阮中宝 杨向军 陈各才 朱莉 李伟 杨兵 欧阳溪 潘少辉 |
| 作者单位： | 1. 苏州大学附属第一医院心内科,江苏省苏州市,215006 2. 泰州市人民医院心内科,江苏省泰州市,225300 3. 江苏省北科生物科技有限公司,江苏省泰州市,225300 |
| 摘 要： | 背景:5-氮杂胞苷能诱导人脐带间充质干细胞分化为心肌细胞。目的:以5-氮杂胞苷诱导人脐带间充质干细胞分化为心肌细胞。方法:采用贴壁培养法分离、纯化人脐带间充质干细胞,以5-氮杂胞苷诱导第3代人脐带间充质干细胞分化为心肌样细胞。结果与结论:诱导前人脐带间充质干细胞呈典型的梭形;诱导后一二周细胞体积变大,三四周后相邻细胞间胞膜有接触,逐渐相连呈肌管状,细胞胞质内可见细丝样结构。诱导4周后免疫组织化学鉴定人脐带间充质干细胞cTnI表达阳性,未诱导细胞cTnI表达阴性;诱导组Nkx2.5、GATA4mRNA表达水平较未诱导组显著增加(P<0.05)。提示5-氮杂胞苷可能通过调控GATA4、Nkx2.5基因的表达促进人脐带间充质干细胞分化为心肌样细胞,并促进其成熟。 |
| 关 键 词： | 脐带 间充质干细胞 细胞分化 心肌细胞 5-氮杂胞苷 |
5-azacytidine induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyocytes |
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| Ruan Zhong-bao,Yang Xiang-jun,Chen Ge-cai,Zhu Li,Li Wei,Yang Bing,Ouyang Xi,Pan Shao-hui.5-azacytidine induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyocytes[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(36):6705-6708. | |
| Authors: | Ruan Zhong-bao Yang Xiang-jun Chen Ge-cai Zhu Li Li Wei Yang Bing Ouyang Xi Pan Shao-hui |
| Institution: | Ruan Zhong-bao1,Yang Xiang-jun1,Chen Ge-cai2,Zhu Li2,Li Wei3,Yang Bing3,Ouyang Xi3,Pan Shao-hui3 1Department of Cardiology,the First Affiliated Hospital,Soochow University,Suzhou 215006,Jiangsu Province,China,2Department of Cardiology,Taizhou People's Hospital,Taizhou 225300,3Jiangsu BeiKe Bio-Technology |
| Abstract: | BACKGROUND:5-azacytidine(5-aza) can induce the differentiation of human umbilical cord derived mesenchymal stem cells(hUCMSCs) into cardiomyocytes.OBJECTIVE:To investigate the cellular mechanism underlying the differentiation of hUCMSCs into cardiomyocytes induces by 5-aza.METHODS:hUCMSCs were isolated and purified by adherent method.hUCMSCs at passage 3 were induced to differentiate into cardiomyocytes by 5-aza.RESULTS AND CONCLUSION:Spindle-shaped hUCMSCs were presented from human umbilical cord tissue by... |
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