| 骨碎补总黄酮可促进兔骨髓间充质干细胞的增殖和分化 |
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| 引用本文： | 刘伟,赵劲民,苏伟,李晓峰,秦义武. 骨碎补总黄酮可促进兔骨髓间充质干细胞的增殖和分化[J]. 中国组织工程研究与临床康复, 2011, 15(32): 6021-6026. DOI: 10.3969/j.issn.1673-8225.2011.32.030 |
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| 作者姓名： | 刘伟 赵劲民 苏伟 李晓峰 秦义武 |
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| 作者单位： | 广西医科大学第一附属医院创伤骨科手外科,广西壮族自治区南宁市,530021 |
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| 基金项目： | 广西自然科学基金重点项目,课题名称:骨碎补总黄酮诱导骨髓间充质干细胞分化为成骨细胞的蛋白质组学研究 |
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| 摘 要： | 背景:骨碎补促进骨损伤愈合疗效确切,但具体作用机制尚不清楚。目的:观察骨碎补总黄酮对兔骨髓间充质干细胞增殖、分化的影响。方法:冲洗兔股骨和胫骨骨髓腔获取骨髓,采用密度梯度离心法联合贴壁培养法体外纯化扩增兔骨髓间充质干细胞;以四甲基偶氮唑蓝法测定不同浓度骨碎总黄酮对兔骨髓间充质干细胞增殖的影响;经骨碎补总黄酮体外诱导后,行扫描电镜形态学观察,碱性磷酸酶染色、茜素红染色和VonKossa银染色了解细胞钙化情况。结果与结论:兔骨髓间充质干细胞可以通过密度梯度离心法联合贴壁培养法扩增和纯化;含骨碎补总黄酮血清培养后,四甲基偶氮唑蓝测定结果显示,骨碎补总黄酮浓度为10-6mmol/L时可明显促进兔骨髓间充质干细胞增殖。经骨碎补总黄酮诱导液诱导,扫描电镜下可见成骨细胞样形态和钙结节形成;细胞碱性磷酸酶染色、钙结节茜素红染色和VonKossa银染色均呈阳性,提示骨碎补总黄酮可促进兔骨髓间充质干细胞增殖和分化。
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| 关 键 词： | 骨碎补总黄酮 骨髓间充质干细胞 增殖 分化 兔 |
Effects of assemble flavone of rhizoma drynariae on proliferation and differentiation of rabbit bone marrow mesenchymal stem cells |
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| Liu Wei,Zhao Jin-min,Su Wei,Li Xiao-feng,Qin Yi-wu. Effects of assemble flavone of rhizoma drynariae on proliferation and differentiation of rabbit bone marrow mesenchymal stem cells[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(32): 6021-6026. DOI: 10.3969/j.issn.1673-8225.2011.32.030 |
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| Authors: | Liu Wei Zhao Jin-min Su Wei Li Xiao-feng Qin Yi-wu |
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| Affiliation: | Liu Wei,Zhao Jin-min,Su Wei,Li Xiao-feng,Qin Yi-wu Department of Orthopedic Trauma and Hand Surgery,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China |
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| Abstract: | BACKGROUND: Rhizoma drynariae is commonly used in orthopedics and showing an accurate effect; however, it specific mechanisms are not very clear. OBJECTIVE: To explore the influences of assemble flavone of rhizoma drynariae on proliferation and differentiation of rabbit bone marrow mesenchymal stem cells (BMSCs). METHODS: The rabbit BMSCs were isolated from femur and tibia bone marrow by combination of gradient centrifugation and different adherent method, then proliferated and purified in vitro. The influe... |
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