GM-1对新生大鼠神经干细胞增殖和分化的影响

目的:观察不同剂量神经节苷脂(GM—1)对神经干细胞增殖和分化的影响。方法:从新生大鼠海马组织分离出神经干细胞,分别培养于基础培养液(阴性对照组) ,加入三种不同剂量GM—1(实验组)及含有b FGF(阳性对照组)的神经干细胞培养液。采用神经干细胞球直接计数法测定细胞的增殖能力,并应用免疫细胞化学技术对神经干细胞分化情况进行鉴定。结果:3个实验组神经干细胞的增殖能力较阳性对照组无明显差异,较阴性对照组差异显著,贴壁培养细胞分化发现3个实验组分化能力明显低于阳性对照组。结论:GM—1对新生鼠神经干细胞的增殖起促进作用,对神经干细胞无明显的诱导分化作用。

Effects of GM-1 on the proliferation and differentiation of hippocampal neural stem cells in newborn rats

Objective:To observe the effects of different dose of GM-1 on the proliferation and differentiation of the hippocampal neural stem ca lls. Methods: The neural stem cells isolated from the hippocampus of the newborn rats were cultured in the medium(negative-control group: DMEM/F12 , B27 and glutamate, pH=7.0), the medium containing bFGF, and the medium contain ing different dosage of GM-1, respectively. The proliferation of different group s was examined by counting the number of neurospheres and the differentiation of neural stem cells was identified by the immuncytochemical technique. Re sults: There was no significant difference in the number of neurospheres b etween three experimental groups and the positive-control group, but the differe nce between experimental groups and the negative-control group was significant. Conclusion: GM-1 has effects on the proliferation of the neural stem cells, while it has no obvious influence on the differentiation of the neural stem cells.