卵巢癌患者瘤组织某些肿瘤相关基因表达、DNA倍性与肿瘤转移的关系

目的 探讨卵巢癌患者肿瘤组织相关基因编码蛋白表达与肿瘤转移的关系.方法 采用流式细胞术对32例卵巢癌患者肿瘤组织的p53V 、CD44v5 、CD44v6 、cerbB-2 、nm23 细胞百分率和DNA含量进行了检测和分析.结果 卵巢癌患者p53V 、CD44v5 、CD44v6 、cerbB-2 的百分率显著升高(P＜0.01或P＜0.05),肿瘤转移者进一步升高;nm23 细胞表达率却显著降低(P＜0.05),肿瘤转移时进一步降低(P＜0.05).肿瘤组织DNA倍体类型不同,p53V 、CD44v5 、CD44v6 、cerbB-2 、nm23 细胞表达率明显不同.结论 卵巢癌患者肿瘤组织肿瘤相关基因编码蛋白表达率、DNA倍性和肿瘤转移之间的关系十分密切.这些结果提示,肿瘤组织肿瘤相关基因表达水平和DNA倍体性,不仅可以作为卵巢癌患者病情发展和转移的指征,而且还可以作为肿瘤恶性度和患者预后判断的重要指标.

The Relativity between Oncogene Coding Protein Expression and DNA Ploidy Measurement in Tumor Tissue and Tumor Metastasis from Patients with Ovarian Cancer

Objective To approach the relationship between oncogene coding protein expression in tumor tissue and tumor metastasis from patients with ovarian cancer.Methods The percentage of p53V ,CD44v5 ,CD44v6 ,cerbB-2 ,nm23 cells and DNA content in the tumor tissue from 32 patients with ovarian cancer were measured and analyzed by flow cytometry.Results The percentage of p53V ,CD44v5 ,CD44v6 and cerbB-2 cells in tumor tissue were significantly higher than those in normal control(P<0.01 or P<0.05).As tumor metastasis,these parameters were gradu-ally increased.The percentage of nm23 cells in tumor tissue was significantly lower than that in normal control(P<0.05).As tumor metastasis,this parameter was gradually decreased.These parameter were significant differed followed by differ-ence of DNA ploid types in tumor tissue.Conclusions There is marked relationship among the expression rate of oncogene coding protein,DNA ploidy in tumor tissue and tumor metastasis in patients with ovarian cancer.Therefore,these parameter may be regarded as not only a marker of development and metastasis of ovarian cancer,but also a reference for the judgment of malignant degree of the cancer and the prognosis.