肝细胞生长因子对培养的视网膜色素上皮 细胞移行及增生的作用

目的研究肝细胞生长因子(hepatocyte growth factor, HGF)对视网膜色素上皮 (retinal pigment epitheliaum, RPE) 细胞的促增生和促移行作用。方法在无血清培养液培养的人RPE细胞中分别加入1、2、10、50、100 μg/L不同浓度的HGF，四唑盐比色法(methyl thiazolyl tetrazolium, MTT)测定促细胞增生情况； 采用RPE细胞损伤愈合模型，在无血清培养液中分别加入1、2、10、50、100 μg/L 不同浓度的HGF，培养20 h后计数进入刮痕区的细胞，观察HGF对RPE细胞移行的作用。结果HGF浓度处于10 ～100 μg/L时对RPE细胞具有促增生作用(P<0.05或P<0.01), 增生率为18.2 %～34.8 %，其中浓度为50 μg/L作 用3 d达到最大促增生效果(P<0.01），增生率为32.8 %；HGF可明显促进RPE细胞移行，促移行能力分别为113.0 %(10 μg/L), 91.7 %(50 μg/L) 和50.3 %(100 μg/ L )。浓度自2 μg/L开始出现促细胞移行作用(P<0.05)，促移行能力为9.3 %。结论HGF可促进RPE细胞增生和移行,是RPE细胞的有丝分裂原和强力的促移行因子。(中华眼底病杂志,2001,17:307-310)

Effect of hepatocyte growth factor on migration and proliferation in cultured retinal pigment epithelial cells

ObjectiveTo investigate the effect of HGF on proliferation and migration in cultured human RPE cells.MethodsHuman RPE cells cultured in serum-free medium were treated with HGF(1，2，10，50，100 μg/L), and MT T assay was used to detect the growth of the cells; an in vitro wound healing model was used to count the number of cells that had entered the denudate area in RPE mig ration treated with HGF (1，2，10，50，100 μg/L) after 20 h.ResultsHGF(10,50,100 μg/L) increased proliferation rates of cultur ed human RPE (18.2 % to 34.8 %), and at a concentration of 50 μg/L on day 3 HGF induced the maximal increase of proliferation (P<0.01）; HGF showed effects on migration of 9.3 %, 113.0 %, 91.7 % and 50.3 % at the concentrations of 2,10,50, 100 μg/L, respectively. HGF stimulated t he strongest migratory response in RPE cells at a concentration of 10 μg/L (113 %).ConclusionHGF can induce the proliferation and obvious migration of RPE cells, consequently HGF was a mitogen and potent migratory factor for human cultured RPE cells. (Chin J Ocul Fundus Dis, 2001,17:307-310)