非心脏缺血预处理对兔未成熟心肌的保护作用

目的　研究非心脏缺血预处理 ( IP)对未成熟心肌的保护作用 ,探讨未成熟心肌的保护方法。　方法　采用心脏缺血预处理 ( MIP)、双下肢缺血预处理 ( DL IP)和肾缺血预处理 ( RIP)兔 L angendorff灌注模型 ,比较 3种方法对缺血 -再灌注 ( IR)未成熟心肌损伤的效应。将 2 4只兔分为 4组 ,IR组 ,MIP组 ,DL IP组和 RIP组 ,每组 6只。测定左心室功能恢复、心肌含水量 ( MWC)、乳酸脱氢酶 ( L DH)漏出量、血清肌酸激酶 ( CK)漏出量、心肌三磷酸腺苷 ( ATP)、丙二醛( MDA)含量和超氧化物歧化酶 ( SOD)活性等指标。　结果　 MIP组、DL IP组和 RIP组左心室功能恢复指标、ATP含量和 SOD活性优于 IR组 ( P<0 .0 5 ) ,MWC低于 IR组 ( P<0 .0 5 ) ,L DH、CK漏出率和 MDA含量均低于 IR组 ( P<0 .0 1)。　结论　非心脏 IP对未成熟心肌具有明显的保护作用 ,与 MIP相同 ,可诱发同等效应的心肌保护作用。

Effect of Non-myocardial Ischemic Preconditioning on Immature Myocardial Protection in Neonatal Rabbits

Objective To study the immature myocardial protection effects with non myocardial ischemic preconditioning and inquire the methods of immature myocardial protection. Methods The effects of immature myocardial protection were observed with myocardial ischemic preconditioning(MIP), double lower limbs ischemic preconditioning (DLIP) and renal ischemic preconditioning (RIP). Twenty four rabbits were divided into four groups, ischemic reperfusion (IR) group,MIP group, DLIP group, and RIP group. Each group includes 6 rabbits. The left ventricular function recovery , myocardial water content (MWC), lactate dehydrogenase (LDH) and creatine kinase (CK) leakage , malondialdehyde (MDA) and adenosine triphosphate (ATP) content and superoxide dismutase (SOD) activity were tested. Results The recovery of postischemic heart function ,ATP content,SOD activity in MIP group, DLIP group and RIP group were higher than those of IR group.MWC, LDH, CK leakage, and MDA content in MIP group, DLIP group and RIP group were lower than those of IR group. Conclusion DLIP and RIP has the same protection to immature myocardium as MIP.