突变型乙肝病毒前C-C基因疫苗免疫BALB/C小鼠的研究

目的 了解乙肝病毒前C-C基因疫苗(VEC)的突变型VE2、VE4免疫BALB/c小鼠后诱发特异性体液和细胞免疫的效果.方法 分别用突变型和非突变型DNA疫苗免疫BALB/c小鼠,ELISA法检测小鼠抗HBc、抗HBelgG,免疫第28天取小鼠脾细胞用酶免疫斑点(ELISPOt)方法和CTL杀伤试验检测特异性细胞免疫功能.结果 VE2、VFA组抗HBe水平高于VEC组,抗HBc水平差异无统计学意义.3种质粒均能引发特异性细胞免疫反应,VE4、VE2强于VEC;联用VM7免疫后能使VEC、VE2、VE4特异性细胞免疫反应增强.结论 突变型前C-C基因疫苗在诱导BALB/c小鼠特异性体液和细胞免疫方面优于突变前.γ干扰素基因可作为基因佐剂增强HBV DNA疫苗诱导的特异性细胞免疫反应.

Immune effects of mutated Hepatitis B Virus precore-core DNA vaccines in mice

Objective To observe the immune effect of DNA vaccines encoding mutated HBV pre-c/c gene(VE2,VFA) in mice. Methods Three kinds of plasmid VEC(DNA vaccines encoding HBV pre-c/c gene),VE2 and VE4 were injected into the thigh muscles of different group of BALB/c mice. Blood and splenocytes from mice were isolated at 4 weeks after immunization. We also have mouse groups immunized with three of these plasmid combined with IFN-γ gene plasmids. The anti-HBc and anti-HBe antibody in peripheral blood in mice were detected by enzyme linked immunusorbent assay( ELISA),antigen-specific cell immune responses were detected by CTL test and enzyme linked immunospot assay( ELISPOt). Results We found that anti-HBe titers of VE2 and VE4 immunizing groups are higher than VEC group( P < 0.05). We also observed that VE2 and VE4 could induce stronger antigen-specific immune responses than VEC and when combined with IFN-γ plasmid,the antigen-specific immune responses are stronger than those without combination immunization in mice (P < 0.05). Conclusions The DNA vaccine VE2 and VE4 could induces stronger antigen-specific immune responses than VEC,and when combined with IFN-γ plasmid,the antigen-specific immune responses are improved in mice.