| 化学发光法与酶联免疫吸附法筛查HIV抗体的效果比较 |
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| 引用本文： | 石向东,甘永霞,王晓辉,张燕,陈琳. 化学发光法与酶联免疫吸附法筛查HIV抗体的效果比较[J]. 中国热带医学, 2010, 10(5): 532-533 |
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| 作者姓名： | 石向东 甘永霞 王晓辉 张燕 陈琳 |
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| 作者单位： | 深圳市疾病预防控制中心艾滋病防制科,广东,深圳,518020 |
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| 摘 要： | 目的比较化学发光法(ECLIA)和酶联免疫吸附法(ELISA)筛查HIV抗体效果的差异。方法用化学发光法和酶联免疫吸附法分别对807份血清样本(其中包括239份WB确证阳性血清和568份WB确证阴性血清)进行平行操作筛查HIV抗体,并比较检测结果。结果两种方法的敏感性均为100%,特异性和功效率均高于95%。结论 ECLIA法和ELISA法均具有较高的敏感性和特异性,可广泛应用于HIV抗体筛查。
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| 关 键 词： | HIV抗体 化学发光法 酶联免疫吸附法 |
Comparison of the result of different HIV antibody screening method as chemiluminescence and ELISA |
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| Affiliation: | SHI Xiang-dong,GAN Yong-xia,WANG Xiao-hui,et al. (Shenzhen Municipal Center for Disease Control and Prevention, Shenzhen 518020,Guangdong,p. R. China) |
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| Abstract: | Ahn To compare the results of screening of antibodies to HIV by chemiluminescence （ECLIA）and ELISA. Methods There 239 HIV antibody positive samples and 568 HIV antibody negative samples were tested by ECLIA and ELISA. Results The sensitivity of two methods were all 100% and the specificity and efficiency all exceeded 95%. Conclusion The sensitivity and specificity of ECLIA and ELISA are high and suitable for detection of antibodies to HIV. |
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| Keywords: | HIV antibody Chemiluminescence ELISA |
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