PT和APTT标本留存时间与结果稳定性关系

目的探讨标本的待测时间对凝血酶原时间（PT）和活化部分促凝血酶原时间（APTT）试验结果的影响。方法：在室温下分别于采样后即刻、2、4、6、24h测定了40例志愿者的PT、AFTT，同时进行了血浆留存和全血留存结果的比较。结果：6h内无论全血留存标本还是血浆留存标本，其PT值和APPT值与初始值相比均无明显变化（P＞0．05）；24h全血留存标本PT值略升高（P＞0．05），血浆留存标本较初始值延长14％，有显著差异（P＜0．05）。APTT值两类标本在6h内与初始值相比也无明显变化（P＞0．05），24h均明显延长（P＜0．05）。结论：血浆留存标本或全血留存标本PT或APTT测定宜在采集标本后6h内完成。

Relations Between Time of Stored Specimens of PT or APTT and Stability of Test Results

Objective To explore the time of specimens waiting for the test and its iafluence onthe test results of prothrombin time (PT) and activated partial thromboplastin time(APTT). Methods:The PT and APTT in 40 volunteers were determined at room temperature immediately afer the specimenswere taken, at 2, 4, 6 and 24 h, respectively. Meanwhile, a comparison between the stored plasma andthe stored whole blood was made. ResultS: In comparison with the initial values of the stored whole bloodspecimens and plasma specimens, there were no significant changes in PT and APTT values within 6 h(P > 0.05). The PT value of the stored whole blood specimens increased slightly at 24 h (P > 0 .05 ).As compared with the initial values, the PT values of the stored plasma specimens were prolonged by 14percent, and they had significant difference (P < 0.05 ). In comparison with the initial values, theAPTT values of the two kinds of specimens had no significant changes at 6 h(P > 0 .05 ). They were allprolonged significantly at 24 h(P < 0.05 ). Conclusion: It is better for the stored plasma or the wholeblood and PT or APTT to be tested after the specimens being collected within 6 hours.