厚朴种质资源评价与利用研究

目的:选择优良种质资源,为厚朴品质改良的研究提供依据,以满足中药现代化、产业化和国际化的要求。方法:收集7省13个厚朴主产区种源的种子,在浙江景宁同一地点种植,于7年生时采样,用高效液相色谱法(HPLC)测定有效成分。结果:不同种源厚朴酚类含量存在极显著的差异,同一种源内个体间的差异更大。结论:湖北五峰、鹤峰和恩施3个种源厚朴质量明显优于其它种源,厚朴酚、和厚朴酚、厚朴酚类总量都很高,推广应用这3个优良种源,是当前提高厚朴质量最有效的途径。厚朴种源内个体间酚类含量存在巨大差异,离中药现代化、国际化“质量稳定、可控”的要求,仍然存在较大的差距,但这种差异可为厚朴选育种提供物质基础和巨大潜力。建议在育种工作中加强对优良单株的选择。

Quality Evaluation and Utilization of Germplasm Resources of Magnolia officinalis

Objectives: To lay a theoretical foundation for studies on strategies for improvement of Magnolia officinalis and select superior germplasm resources to meet the demand for modemization, industrialization and internationalization of Chinese medicine. Methods: Seeds of Magnolia offcinalis from 13 main habitats of 7 provinces were collected and strewn in a place of Jingning County, Zhejiang. At the age of seven, 195 samples were collected from the same height of the trunk of 15 individual trees of each provenance, and assayed for effective ingredients with HPLC. Results: Differences in the content of phenols were significant among the seed sources and even greater among individuals within a seed source. Conclusions:3 seed sources viz. Wufeng, Hefeng and Enshi of Hubei were obviously superior to other seed sources on account of high contents of magnolol, honokoiol and total phenols. Extension and applcation of these 3 seed sources is an effective path leading to quality improvement of Magnolia offidnalis . With great differences in the content of phenols existing among individuals within each source, there is a big gap between production of medicinal materials by merely using superior seed sources of Magnolia offidnalis and the demand of stable and controllable quality for modernization and internationalization of Chinese medicine. But the great difference has laid a material foundation and brought about a great potential for genetic improvement of Magnolia offcinalis. Therefore, the superior individuals within a superior seed source are an excellent material for the breeding of Magnolia officinalis.