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严重少精子症患者与正常生育男性精浆蛋白质群比较分析
引用本文:白洁,孙玲,朱红,张立文,马俊龙,丛玉隆.严重少精子症患者与正常生育男性精浆蛋白质群比较分析[J].中华男科学杂志,2008,14(4):298-302.
作者姓名:白洁  孙玲  朱红  张立文  马俊龙  丛玉隆
作者单位:1. 中国人民解放军总医院临床检验科,北京,100853
2. 南方医科大学附属南方医院生殖医学中心,广东,广州,510515
摘    要:目的:探讨严重少精子症和正常生育男性精浆蛋白质群的差异性。方法:11例正常健康已生育的自愿者(正常组)和6例严重少精子症男性精液标本通过Percoll分离获取精浆。采用SELDI-TOF-MS,经CM10芯片捕获蛋白质并用TOF-MS对蛋白质进行检测,获得各样本的蛋白质指纹图谱,经过归一化处理后进行组间比较。结果:严重少精子症组与正常组比较仅有2种低丰度蛋白质表达存在差异,与非梗阻性无精子症组比较差异蛋白质达15种。蛋白质荷比(m/z)分别为7196.058、7547.610、5780.493、7059.844、7409.589、5379.173、10778.810的7种蛋白质是严重少精子症、正常组与非梗阻性无精子症组的共同差异蛋白质,除后两者在非梗阻性无精子症中含量升高外,其余含量均降低。结论:严重少精子症的精浆蛋白质群与正常组差异较小,即两者的精浆蛋白质组成较为相似,但二者均与非梗阻性无精子症存在显著差异。提示严重少精子症和非梗阻性无精子症的发生机制不同,并非仅是遗传因素量的累加。

关 键 词:严重少精子症  精浆  差异蛋白质  SELDI-TOF-MS
文章编号:1009-3591(2008)04-0298-05
修稿时间:2007年4月25日

Protein Compositions in the Seminal Plasma Are Similar Between Severe Oligospermic and Healthy Fertile Men
BAI Jie,SUN Ling,ZHU Hong,ZHANG Li-wen,MA Jun-long,CONG Yu-long.Protein Compositions in the Seminal Plasma Are Similar Between Severe Oligospermic and Healthy Fertile Men[J].National Journal of Andrology,2008,14(4):298-302.
Authors:BAI Jie  SUN Ling  ZHU Hong  ZHANG Li-wen  MA Jun-long  CONG Yu-long
Institution:Clinical Lab of PLA General Hospital, Beijing 100853, China. jiebai69@tom.com
Abstract:OBJECTIVE: To analyse the variability of proteins in the seminal plasma of severe oligospermic and healthy fertile men. METHODS: Spermatic fluid samples were collected from 11 healthy fertile men and 6 severe oligospermic male volunteers and tested by SELDI-TOF-MS with the CM10 protein chip to get the protein spectra maps, which were automatically treated with the special softwares of Ciphergen Inc. RESULTS: The mean peak heights of 2 lower-abundance proteins expressed in the seminal plasma of the severe oligospermic men were statistically different from the healthy fertile males (P<0.05). Fifteen different proteins existed between the nonobstructive azoospermic and the severe oligospermic group, 7 of which, with m/z of 7,196.058, 7,547.610, 5,780.493, 7,059.844, 7,409.589, 5,379.173 and 10,778.810, also between the non-obstructive azoospermic and the healthy fertile males (P<0.05). Except the latter two, the contents of the other 5 proteins were decreased in the non-obstructive azoospermic men (P<0.05). CONCLUSION: The finger prints of the seminal plasma proteins of the severe oligospermic group were similar to those of the healthy fertile males, both significantly different from the non-obstructive azoospermic men. It is suggested that pathogenesis mechanisms differ exist between non-obstructive azoospermia and severe oligospermia but are not the simple accumulation of genetic factors.
Keywords:severe oligospermia  seminal plasma  different proteins  SELDI-TOF-MS
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