染料木索预防大鼠肝纤维化形成的实验研究

目的研究染料木素（genistein,G）对实验性肝纤维化大鼠的预防效果。方法SD雄性大鼠50只，按随机数字表法分为生理盐水对照组、模型组、治疗组、染料木素对照组。模型组和治疗组采用硫代乙酰胺（TAA）腹腔注射3个月制造肝纤维化模型。造模结束后采用ELISA法检测各组血清Ⅲ型前胶原氨基端肽（PⅢNP）、层粘连蛋白（LN）含量，各组肝组织行HE染色病理检查。结果治疗组较模型组血清PⅢNP和LN水平均降低（均P〈0．05），肝脏纤维组织沉积减少（P〈0．05）。结论染料木素对大鼠肝纤维化形成具有一定的预防作用。

Genistein prevents experimental liver fibrosis in tats

Objective TO investigate the preventive effect of genistein on experimental liver fibrosis in rats. Methods Liver fibrosis was induced by intraperitoneal injection of thioacetamide （TAA） in male SD rats for three months. Fifty SD rats were divided into 4 groups： normal control （no TAA and genistrin was given）, genistein control group （normal rats, genistein was given）, model group （fibrosis was induced, but no genistein was given） and treatment group （fibrosis induced and genistein given）. The procollagen type Ⅲ N-peptide （P Ⅲ NP） and Laminin （LN） in the serum were measured by ELISA, the fibrosis level of liver samples were histopathologically observed by HE stain. Results The levels of serum P ⅢNP, LN and the accumulation of fibrous tissue in treatment group were lower than those in model group （P〈 0.05）. Conclusion Genistein can prevent experimental liver fibrosis in rats.