新疆红树莓果实不同有效部位提取及其抗氧化活性测定

目的测定树莓果实不同提取部位总酚含量并比较不同提取部位抗氧化活性。方法树莓果实95%乙醇提取物经石油醚脱脂后,依次用乙酸乙酯、正丁醇萃取,对得到的各提取部位浸膏,①以没食子酸为标准品进行总酚含量测定,同时用相对标准偏差(RSD)表示稳定性;②采用1,1-二苯-2-苦肼基和羟自由基清除能力实验测定抗氧化活性。结果①Folin-Ciocalteu比色法具有精密度高、稳定性和重现性好等优点;②各提取部位物总酚含量从GAE10.56mg/g到96.76mg/g,其中以乙酸乙酯部位总酚含量最高(GAE96.76mg/g);③抗氧化实验中,树莓不同提取物均显示了较好的抗氧化活性,其中以乙酸乙酯部位抗氧化活性最高。结论树莓是良好的天然抗氧化剂。

Determination of anti-oxidative activity of different fractions of Xinjiang red raspberry fruits extract

Objective To determination total phenolics content of raspberry different polar solvent extracts was measured by Folin-Ciocaheu method. Methods Red raspberry fruits were extracted with 95% ethanol, defatted with petroleum ether, and then partitioned with petroleum ether, ethyl acetate and butanol successively. Total phenolic content of each part was measured by Folin-Ciocalteu method and calculated as gallic acid equivalents (GAE). Stability of the method were assayed with RSD (relative standard deviation) . Antioxidant activities of the different extractions were evaluated with DPPH·and OH·scavenging assay.Results (1)The mehtod had high quality of precision,stability,repetition rate. (2)Total phenolic contents were varied from GAE 10.56mg/g to GAE 96.76mg/g as gallic acid equivalent, and ethyl acetate extracts had the highest total phenolic content (GAE 96.76mg/g). (3)The crude extracts and different soluble fractions showed good autioxidative activities. ConclusionRaspberry could be good resource of natural antioxidant.