| 应用蛋白质组学技术筛选胰腺癌血清肿瘤标志物 |
| |
| 引用本文： | 徐树建,徐泽宽,王富强,朱 毅,苗 毅.应用蛋白质组学技术筛选胰腺癌血清肿瘤标志物[J].南京医科大学学报,2009,29(4):534-538. |
| |
| 作者姓名： | 徐树建 徐泽宽 王富强 朱 毅 苗 毅 |
| |
| 作者单位： | 徐树建,徐泽宽,苗毅,XU Shu-jian,XU Ze-kuan,MIAO Yi(南京医科大学第一附属医院胆胰外科,江苏,南京,210029);王富强,WANG Fu-qiang(南京医科大学组织与胚胎学系生殖医学实验室,江苏,南京210029);朱毅,ZHU Yi(南京医科大学第一附属医院普通外科实验室,江苏,南京,210029)
|
| |
| 基金项目： | 江苏省兴卫工程重点人才基金 |
| |
| 摘 要： | 目的:比较胰腺癌患者与健康志愿者血清蛋白的差异以寻找候选血清肿瘤标志物,为胰腺癌早期诊断提供依据.方法:通过免疫亲合柱去除丰度最高的14种血清蛋白,用双向电泳分离蛋白并比较差异.选取在胰腺癌患者血清中明显差异表达蛋白点,行质谱鉴定和生物信息学分析.结果:建立了稳定性、重复性良好的凝胶蛋白图谱.对筛选出的在胰腺癌患者血清中明显差异表达的78个蛋白点,共有54个蛋白点被成功鉴定,其中胰腺癌患者血清中高表达者29个,低表达者25个.结论:胰腺癌患者、健康志愿者血清蛋白之间存在明显差异,通过蛋白质组学方法可筛选并鉴定出这些差异蛋白,后者有望为早期诊断胰腺癌提供依据.
|
| 关 键 词： | 胰腺癌 蛋白质组学 二维凝胶电泳 肿瘤标志物 |
| 收稿时间： | 2008/10/21 0:00:00 |
Screening serum biomarkers for pancreatic cancer by serum proteomics techniques |
| |
| XU Shu-jian,XU Ze-kuan,WANG Fu-qiang,ZHU Yi and MIAO Yi.Screening serum biomarkers for pancreatic cancer by serum proteomics techniques[J].Acta Universitatis Medicinalis Nanjing,2009,29(4):534-538. |
| |
| Authors: | XU Shu-jian XU Ze-kuan WANG Fu-qiang ZHU Yi and MIAO Yi |
| |
| Institution: | Department of Pancreas Surgery;the First Affiliated Hospital of NJMU;Nanjing 210029;1Laboratory of Repro-ductive Medicine;Department of Histology and Embryology;NJMU;Nanjing;210029;2Laboratory of General Surgery;210029 |
| |
| Abstract: | Objective:To find potential molecular targets for pancreatic cancer diagnosis by comparing and analyzing differential proteins in serum between patients with pancreatic cancer and healthy controls,providing evidence for the early diagnosis of pancreatic cancer.Methods:The protein in serum were separated by two-dimensional gel electrophoresis(2-DE)after the top 14 high abun-dance proteins were removed from serum with the ProteinExtractTM Removal kit.The significantly differentially expressed proteins were an... |
| |
| Keywords: | Pancreatic cancer Proteome Two-dimensional electrophoresis biomarker |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《南京医科大学学报》浏览原始摘要信息 |
| 点击此处可从《南京医科大学学报》下载免费的PDF全文 |
|
