不同产地当归中砷暴露对小鼠肝肾毒性损伤的研究

目的研究不同产地当归中重金属砷暴露对小鼠肝肾的损伤情况,并探讨其毒性.方法35只昆明种成年小鼠随机分为正常对照组、当归组(产地分别为云南、四川和陕西)和雄黄组共5组.小鼠灌胃给药72h 后处死,取血,肝脏和肾脏组织,全自动生化分析仪检测血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、尿素氮(UREA)和肌酐(CREA)含量,观察肝肾组织病理学变化;原子荧光光谱测定肝肾组织中的砷蓄积量;实时荧光定量(RT-PCR)检测砷毒性敏感基因金属硫蛋白-1(Metallothionein,MT-1)mRNA 和肿瘤坏死因子-α(TNF-α)mRNA 的表达水平.结果与对照组相比,雄黄组血清 ALT、AST、CREA 浓度水平和肝肾组织中砷蓄积量显著升高(P<0.05),并可能伴随肝肾功能的损伤, MT-1 mRNA 和 TNF-αmRNA 表达量升高但无统计学意义.当归组只在肝组织中检测到微量砷,肝肾功能病理学检查与正常对照组相似,MT-1 mRNA 和 TNF-αmRNA 表达量无显著升高(P>0.05).结论不同产地当归所含砷的毒性远小于雄黄毒性,高剂量雄黄给药对小鼠肝肾组织可能产生影响,但它们在一定剂量下被用于中药仍然是相对安全的.

The Toxic Injury Study of Arsenic Exposure from Different Origin Angelica Sinensis Exposure in Hepatic and Kidney of Mice

Objective To compare toxic injury of arsenic from difference origin Angelica sinensis exposure in mice liver and kidney,and probe into the related mechanisms.Methods Adult Kunming mice were randomly divided into 5 groups(control group,YN group,SC group,SX group and realgar group) and were given a several intragastric administration of Angelica sinensis and Realgar,respectively.Seventy two hours after administration,serum ALT,AST,UREA and CREA were analyzed by automatic biochemical analyzer to observe histopathologic change in liver and kidney.MT-1 mRNA and TNF-α mRNA were determined by RT-PCR.Results Accumulation of arsenicin liver and kidney of the Realgar group were significantly higher than control group,accompanied by the higher levels of serum ALT,AST,UREA,CREA(P<0.050,may indicate injuries in the liver and kidney.Along with a little elevated MT-1 mRNA and TNF-α mRNA gene expression in Realgar group,but not in other groups(P>0.05).Histology showed no pathological injuries of liver and kidney in three different origin Angelica sinensis groups.Conclusion Difference origin Angelica sinensis are much less acute toxic than Realgar.High dose Realgar may have effect on liver and kidney tissue.The Angelica sinensis are still safe to be used in proprietary Chinese medicines.