Detection of staphylococcal genes directly from cerebrospinal and peritoneal fluid samples using a multiplex polymerase chain reaction |
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Authors: | F. -J. Schmitz M. Steiert B. Hofmann A. C. Fluit J. Verhoef H. -P. Heinz M. E. Jones |
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Affiliation: | (1) Institute for Medical Microbiology and Virology, Heinrich-Heine-University Düsseldorf, Universitätsstraße 1, D-40225 Düsseldorf, Germany;(2) Eijkman-Winkler Institute for Medical Microbiology, University Hospital Utrecht, Heidelberglaan 100, PO Box 85.500, NL-3508 GA Utrecht, The Netherlands |
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Abstract: | A multiplex polymerase chain reaction (PCR) that can simultaneously detect eubacterial isolates and the methicillin-susceptibility of staphylococcal isolates from cerebrospinal and peritoneal fluid samples was compared to conventional microbiological methods. Using conventional methods, bacteria were isolated from 8% (29/350) of the cerebrospinal fluid samples and from 5% (3/60) of the peritoneal fluid samples. All isolates except twoStaphylococcus epidermidis isolates were also detected using the multiplex PCR. Coagulase-negative staphylococci andStaphylococcus aureus were correctly identified using both methods. The multiplex PCR can rapidly and simultaneously detect eubacteria, and the methicillin susceptibility of staphylococci from samples containing 102 cfu/ml of bacteria. |
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