热处理延缓维甲酸诱导P19细胞的生长及向神经元分化

研究热环境对P19细胞生长和RA诱导的向神经元分化的影响，为探讨热环境刺激对神经系统发育的影响提供参考。成熟的P19细胞在热环境的条件下培养1h后，消化接种至培养皿中正常培养，于不同时间点取样分析细胞数目。热环境处理P19细胞后用维甲酸诱导分化4d，随后接种至培养皿中，参照神经元的原代培养方法培养12d，用神经元抗体免疫鉴定，计数不同培养时间NSE、NF阳性神经元在总体细胞中的比例。结果发现接受热处理的P19细胞贴壁不良，生长始终缓慢，说明热环境刺激阻止细胞生长分裂。P19细胞经RA诱导分化后出现类似神经细胞的突起和纤维网络，而热环境导致P19细胞向神经元分化明显迟缓，NF阳性细胞比例增加。

Thermotreatment slowed down the growth and differentiation of p19 cells into neuron-like cells induced by retinoic acid

To investigate the effect of heat incubation on the growth of P19 cells and neuronal differentiation of P19 cells induced by retinoic acid (RA). P19 cells were incubated at 43 C for 1h, and growth curve was recorded. After heat incubation, P19 cells were exposed to RA for 4 days and cultured in neural medium for 12 days. Differentiated cells were identified with neuronal antibodies. Most of RA-induced P19 cells differentiated into neuron-like cells with the formation of neurite networks after cultured for 12d in vitro. Heat treatment before RA induction resulted in the decrease of the ratio of NSE positive as well as the increase of NF positive. We propose that heat treatment significantly suppressed the growth and retarded the neuronal differentiation of P19 cells induced by RA.