| 不同靶点的反义bcl—2寡核苷酸对白血病细胞足叶乙甙敏感性影响的研究 |
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| 引用本文： | 雷小勇,张洹.不同靶点的反义bcl—2寡核苷酸对白血病细胞足叶乙甙敏感性影响的研究[J].中国临床药理学与治疗学,2001,6(1):1-4. |
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| 作者姓名： | 雷小勇 张洹 |
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| 作者单位： | 暨南大学医学院血液病研究室，广州 510632 |
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| 基金项目： | 国家自然科学基金(No3987061)和广东省自然科学基金(No980710)资助。 |
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| 摘 要： | 目的探讨针对bcl-2mRNA蛋白编码区的反义寡核苷酸对足叶乙甙诱导HL-60和K562细胞凋亡的影响。方法应用MTT法和流式细胞仪检测反义寡核苷酸与足叶乙甙联合作用的HL60和K562细胞中bcl-2蛋白表达和细胞凋亡及足叶乙甙IC50值。结果10μmol
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| 关 键 词： | 反义核酸 bcl－2 白血病细胞 足叶乙甙 药物敏感性 |
| 修稿时间： | 2000年11月22 |
Effect of bcl-2 antisense
oligonucleotide targeting different region of bcl-2 mRNA on drug-sensitivity of leukemia
cells |
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| LEI Xiao-Yong,ZHANG Huan.Effect of bcl-2 antisense
oligonucleotide targeting different region of bcl-2 mRNA on drug-sensitivity of leukemia
cells[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2001,6(1):1-4. |
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| Authors: | LEI Xiao-Yong ZHANG Huan |
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| Abstract: | Aim To explore the effects of bcl-2 antisense oligonucleotide targeting the coding region of the bcl-2 mRNA on apoptosis induced by Vp16 in HL60 and K562 cells. Methods Drug sensitivity was compared by MTT cytotoxicity assay, and expression of bcl-2 protein and apototic cells were assayed by flow cytometry. Results The bcl-2 antisense oligonucleotide of 10 μmol · L- 1 combined with etoposide inhibited expression of bcl-2 protein, increased apoptosis in HL60 and K562
cells and decreased IC50 of etoposide; The antisense oligonucleotide targeting the coding region of the bcl-2 mRNA had stronger effection than the antisense oligonucleotide targeting the translation initiation. Conclusion The bcl-2 antisense oligonucleotide targeting the coding region of the bcl-2 mRNA can enhances etoposide induced apoptosis of HL60 and K562 cell. |
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| Keywords: | antisense oligonucleotide bcl_2 leukemia cells etoposide drug_sensitivity |
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