SB203580对小鼠造血组织辐射损伤的保护作用

目的观察SB203580对接受γ射线照射的C57BL/6小鼠造血组织损伤的保护作用。方法小鼠随机分为对照(Ctr)组、照射(IR)组及SB203580给药(SB)组,以6.0 Gy 137Csγ射线全身均匀照射SB组和IR组的C57BL/6小鼠。收集并计数外周血红细胞、白细胞和血小板,以及单侧股骨骨髓有核细胞(BMMNC);骨髓细胞半固体培养法检测BMMNC增殖能力。另外收集小鼠BMMNC,同样分组,IR组与SB组细胞进行1 Gy体外照射,照射之后孵育过夜,酶标仪检测细胞内活性氧(ROS)水平。结果 SB组外周血白细胞、血小板的数量分别比IR组高111.8%和157.7%(P<0.05);SB组BMMNC较IR组高135.4%,CFU-GM高188.5%(P<0.05);SB组BMMNC内ROS较IR组低56.2%(P<0.05)。结论 SB203580对照射造成的小鼠造血组织损伤具有一定的保护作用,这种保护作用可能与其降低照射后细胞内ROS水平有关。

The protective effects of SB203580 on hematopoietic tissue exposed to irradiation

Purpose To investigate the protective effects of SB203580 on hematopoietic tissue of C57 BL/6 mice injured by γ-ray.Methods Male C57 BL/6 mice were randomly divided into control group(Ctr),irradiation group(IR) and SB203580 treatment group(SB).Mice were intraperitoneal injected SB(15 mg/kg)30 minutes ahead of TBI and then given every 2 days for5 times after the TBI.On day11,pe-ripheral blood count and nucleated femoral cell count were performed by blood analyzer.1 ×105 cells were seeded into Methocult GF M3534 methylcellulose medium and colony-forming units-granulocyte and mono-cyte(CFU-GM) were scored on day 6.DCFH as a fluorescent probe assessed the reactive oxygen species(ROS) generation in overnight incubated bone marrow mononuclear cells(BMMNC),which were irradiatedat 1 Gy.Results Transitory and significant changes occurred in the cell amount of BMMNC in the irradiated mice after administration of SB203580: white blood cells,platelets and BMMNC in SB were significantly in-creased by 111.8%,157.7% and 135.4%(P < 0.05).The proliferation suppression of BMMNC in IRgroup was eased by SB203580 injection and CFU-GM in SB group score rises by 118.5%(P <0.05).More-over,ROS gets back to the normal level observed in SB group.Conclusion It was shown that changing withcell count and enhancing of proliferation manifested the protection of SB203580 from radiation induced hematopoietic tissue injury.The protection of SB203580 may attribute to its suppression on ROS generation.