| 左旋多巴诱导帕金森病异动症与纹状体DARPP-32磷酸化的关系 |
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| 引用本文： | 宋璐,马雅萍,刘振国,巴茂文,卞雷斯. 左旋多巴诱导帕金森病异动症与纹状体DARPP-32磷酸化的关系[J]. 中国神经免疫学和神经病学杂志, 2011, 18(1): 1-4. DOI: 10.3969/j.issn.1006-2963.2011.01.001 |
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| 作者姓名： | 宋璐 马雅萍 刘振国 巴茂文 卞雷斯 |
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| 作者单位： | 上海交通大学医学院附属新华医院神经内科,200092 |
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| 基金项目： | 上海市科委纳米技术专项基金资助项目，上海市科委基础研究重点项目，上海市教委科研创新重点项目，上海市白玉兰科技人才基金资助项目 |
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| 摘 要： | 目的观察帕金森病(PD)异动症(LID)大鼠模型纹状体区多巴胺和环磷腺苷调节的磷酸化蛋白-32(DARPP-32)蛋白Thr75位点磷酸化表达数量及表达位点的改变。方法给予PD大鼠模型左旋多巴治疗21d,评估大鼠行为学变化;采用免疫荧光和Western blot检测大鼠纹状体区DARPP-32蛋白Thr75位点磷酸化表达数量和表达部位的改变情况。结果 PD大鼠长期使用左旋多巴出现类似于人类LID行为学表现。免疫荧光结果显示PD组和LID组大鼠损伤侧DARPP-32(Thr75)的表达多位于强啡肽阳性神经元。Western blot结果显示PD组大鼠损伤侧DARPP-32(Thr75)表达为(159.90±7.22)%,与假手术组比较升高(P<0.05);LID组大鼠损伤侧纹状体DARPP-32(Thr75)的表达为(52.60±4.45)%,与假手术组和PD组比较降低(P<0.05)。结论纹状体黑质投射神经元内DARPP-32蛋白Thr75位点磷酸化表达的改变可能参与了LID的发病。
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| 关 键 词： | 帕金森病 左旋多巴 DARPP-32 强啡肽 磷酸化 |
The relationship between levodopa-induced dyskinesia and phosphorylation of DARPP-32 in the striatum in experimental Parkinson disease |
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| SONG Lu,MA Ya-ping,LIU Zhen-guo,BA Mao-wen,BIAN Lei-si. The relationship between levodopa-induced dyskinesia and phosphorylation of DARPP-32 in the striatum in experimental Parkinson disease[J]. Chinese Journal of Neuroimmunology and Neurology, 2011, 18(1): 1-4. DOI: 10.3969/j.issn.1006-2963.2011.01.001 |
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| Authors: | SONG Lu MA Ya-ping LIU Zhen-guo BA Mao-wen BIAN Lei-si |
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| Affiliation: | SONG Lu,MA Ya-ping,LIU Zhen-guo,BA Mao-wen,BIAN Lei-si.Department of Neurology,Xinhua Hospital,Jiaotong University,Shanghai 200092,China |
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| Abstract: | Objective To investigate the changes of DARPP-32 phosphorylation in the striatum in experimental Parkinson disease(PD) rats with levodopa-induced dyskinesia(LID).Methods The PD rats were intraperitoneally treated with levodopa for 21 days.Immunoflourescent stain and Western blot were used to observe the changes of DARPP-32(Thr75) phosphorylation in the striatum.Results After levodopa treatment,PD rats displayed ethological changes,which resembled LID in human.The study of immunoflourescent study revealed th... |
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| Keywords: | DARPP-32 |
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