成人脂肪基质细胞诱导分化为星形胶质细胞的生长特征研究

课题组前期研究发现，应用3-异丁基-1-甲基黄嘌呤为主要成分的诱导剂诱导成人脂肪基质细胞分化形成星形胶质细胞的数量和存活时间仍不理想。通过生长曲线对诱导分化的星形胶质细胞的生长分化状态和特征进行系统观察发现，经含0.5%无水乙醇诱导剂诱导的成人脂肪基质细胞在诱导48h即可出现典型星形胶质细胞形态；随着诱导时间的延长，细胞数量呈现出逐渐下降的趋势；诱导14d时，Nestin、胶质纤维酸性蛋白及S-100阳性表达达高峰，神经元特异性烯醇化酶表达为阴性。说明诱导分化的成熟状态的星形胶质细胞在14d时达高峰，进一步优化14d以后的细胞培养环境可获得发育为具有正常功能，存活时间更长，数量更多的成熟星形胶质细胞。

A novel ethanol-based method to induce differentiation of adipose-derived stromal cells into astrocytes

The quantity and survival time of astrocytes, which were differentiated from adult adipose-derived stromal cells after exposure to an inducer containing 3-isobutyl-1-methylxanthine, have thus far been unsatisfactory. The present study investigated the growth and differentiation characteristics of induced astrocytes by observing their growth curves. After induction for 48 hours with an inducer containing 0.5% ethanol, some adult adult adipose-derived stromal cells displayed typical astrocytic morphology. The cell quantity gradually decreased with prolonged induction time. Nestin, glial fibrillary acidic protein, and S-100 expression reached peak levels at 14 days, but neuron-specific enolase was not expressed. These results suggest that the induced astrocytes reached their peak at 14 days. Further optimization of the culture environment may yield mature astrocytes with normal functions, in greater quantity, and prolonged survival time.