利用α-氰基丙烯酸烷基酯医用胶建立人肝癌裸鼠皮下-肝原位移植瘤模型的实验研究

 目的 使用α-氰基丙烯酸烷基酯建立人肝癌-裸鼠皮下肝原位移植瘤模型。方法 将BEL-7402人肝癌细胞接种于裸鼠皮下，建立皮下模型 。形成皮下移植瘤后再移植于裸鼠肝内，利用α-氰基丙烯酸烷基酯建立肝原位移植瘤模型（间接肝原位移植瘤模型）。结果 成功的利用α-氰基丙烯酸烷基酯建立了间接肝原位移植瘤模型。移植瘤存活率达95.8 %。结论 与传统的肝原位移植瘤模型比较，利用α-氰基丙烯酸烷基酯建立间接肝原位移植瘤模型方法简便，成功率高，便于推广使用。

Study of using α-cyanoacrylate to establish the orthotopic transplantation tumor model from the subcutaneous model of human hepatocellular carcinoma in nude mice

Objective Using α-cyanoacrylate to establish the orthotopic transplantation tumor model from the subcutaneous model of human hepatocellular carcinomas(HCC)in nude mice. Methods The subcu－taneous animal tumor model was established by transplanting BEL-7402 cell into the subcutaneous of nude mice. Then the orthotopic transplantation tumor model was established by injecting the subcutaneous trans－planted tumor into liver(indirect orthotopic model). Results Using α-cyanoacrylate to establish the indirect orthotopic model successfully and the survival rates of the transplantation tumor were 95.8 %. Conclusion It was compared with the traditional methods that the indirct orthotopic transplantation tumor model by using α-cyanoacrylate to establish is an easy method with high successful rate and easy to spread.