实验性角膜新生血管的形态观察

目的观察兔角膜新生血管的形态特点,探讨其发生机制及治疗效果.方法将16只新西兰兔随机分为实验和对照两组,均以75%硝酸银液烧灼兔角膜,实验组于硝酸银烧灼后行β射线照射,观察两组角膜新生血管的生长规律;选期制做新生血管铸型,扫描电镜下比较两组间新生血管形态差异.结果角膜新生血管以典型的芽生方式发生和增殖,经β射线照射后的血管芽形成受到明显的抑制和破坏,新生的血管支干发生萎缩、坏死.结论阻断新生血管的芽生过程可抑制角膜新生血管的形成和发展.

An investigation on morphology of experimental corneal neovascularization

OBJECTIVE: To observe the morphologic character of the corneal neovascularization in experimental rabbits and also compare it with the morphology of that after irradiation with beta-rays. METHODS: A model of new blood vessels on the corneas of New Zealand rabbits was established with silver nitrate burning. The growth character of the neovascularization was compared between the irradiated and non-irradiated groups. The casting of the new blood vessels in different periods was made and the morphologic differences between the two groups were compared under a scanning electron microscope. RESULTS: The corneal new blood vessels occurred and continuously proliferated with the typical method of budding. The formation of buds was obviously inhibited and damaged after irradiation with  or  beta-rays, and the irradiated new branches were also atrophic and necrotic. CONCLUSIONS: Stopping the budding procedure of the corneal neovascularization may inhibit the formation and development of the corneal new blood vessels.