广西130株非O1群霍乱弧菌血清学分型及噬菌体-生物分型研究

目的 将噬菌体－生物分型方法应用到非Ｏ１群霍乱弧菌的分型，以掌握广西地区非Ｏ１群霍乱弧菌血清型别的分布，并对此分型方法进行探讨。方法 收集广西部分地区１３０株非Ｏ１群霍乱弧菌进行血清学分型和噬菌体－生物分型，并用多聚酯链反应（ＰＣＲ）进行霍乱肠毒素检测。结果 在１３０例菌株中有９０株可以分型，占６９．２３％，分布于３４个血清型，腹泻病人菌株可分为１４个血清型，其中以Ｏ２以优势血清型，外环境菌株分布

Study on serotyping and phage-biotyping of 130 V.cholerae non-O1 strains in Guangxi

To understand the distribution of different serotypes of V.cholerae non-O1 in Guangxi and to evaluate the method of phage-bioty ping. Methods Serotyping and phage-biotyping systems were used to examine 130 V. cholerae non-O1 strains collected from some areas in Guangxi, and cholera toxin (CT) was detected by PCR.Results 90 strains (69.23%) were subdivided into 34 serotypes among 130 ones.The strains isolated from diarrhea patients were dividedinto 14 serotypes,with O2 predominant; the strains from environment were dividedinto 31 serotypes,with O1and O32 predominant.107 V.cholerae non-O1 strains were identified 14 phagetypes and 4 biotypes.CT detection of 17 strains by PCR was negative.Conclusion Predominant serotypes from diarrhea patients were different from those from environment,while the results of phage-biotyping were nearly the same .CT negative confirmed that these strains were non-epidemic ones and could not cause epidemic.