六味地黄多糖体外对正常及衰老小鼠脾细胞免疫功能的影响

目的探讨六味地黄多糖免疫药理活性的物质基础。方法运用现代药理学与植物化学紧密配合的方法，从六味地黄汤中逐步分离获得了六味地黄多糖ＣＡ４ ３Ｂ和Ｐ ３。应用［３Ｈ］ ＴｄＲ参入法和溶血空斑实验观察了其体外对小鼠脾细胞免疫功能的作用。结果ＣＡ４ ３Ｂ、Ｐ ３体外单独使用对正常及快速老化模型ＳＡＭＰ８和ＳＡＭＲ１小鼠脾细胞增殖反应有明显的促进作用，但不能增强有丝分裂原ＣｏｎＡ诱导的脾细胞增殖反应，大剂量时有一定的抑制作用。ＣＡ４ ３Ｂ对绵羊红细胞（ＳＲＢＣ）体外诱导的正常小鼠脾抗体形成细胞生成反应亦有明显的促进作用，使抗体形成细胞（ＰＦＣ）的数目明显增加。结论ＣＡ４ ３Ｂ和Ｐ ３可能为具有免疫调节作用的活性多糖。

Effects of polysaccharides from liuwei dihuang decoction on the splenocyte immune function in vitro

AIM To explore the substance basis of immunomodulating components. METHODS Liuwei dihuang polysaccharides, CA43B and P3, were isolated from liuwei dihuang decoction employing the method of close collaboration between chemical and pharmacological studies under the guidance of activity evaluation. The action of immunomodulation of CA43B and P3 on mouse splenocytes was evaluated with splenocyte proliferation and antibody production response by TdR incorperation and plaqueforming cell (PFC) assays, respectively, in vitro. RESULTS Both CA43B and P3 enhanced splenocyte proliferation in normal mouse and senescenceaccelerated mouse (SAM) when used alone, but did not promote splenocyte proliferation induced by Con A. The splenocyte proliferation was inhibited when the doses of CA43B and P3 increased to 180 and 680 mmolL-1 respectively. CA43B promoted the antibody production response induced by sheep red blood cells (SRBC) also, and increased the number of PFC of the splenocytes. CONCLUSION CA43B and P3 are probably active polysaccharides possessing immunomodulating effects.