杏仁核中一氧化氮对睡眠-觉醒的影响

目的研究杏仁核中一氧化氮（ＮＯ）对大鼠睡眠 觉醒的影响，并分析其作用机制。方法多导睡眠描记和杏仁核微量注射。结果一氧化氮合酶抑制剂Ｌ 硝基精氨酸（Ｌ ＮＮＡ）可增加慢波睡眠（ＳＷＳ）和减少觉醒（Ｗ），而一氧化氮（ＮＯ）供体硝普钠（ＳＮＰ）可增加Ｗ、减少ＳＷＳ，并可对抗Ｌ ＮＮＡ的促睡眠效应；ＮＯ前体Ｌ 精氨酸（Ｌ Ａｒｇ）对睡眠 觉醒无直接影响，但可对抗Ｌ ＮＮＡ的促睡眠效应。环磷酸鸟苷（ｃＧＭＰ）具有明显的增加Ｗ和减少ＳＷＳ效应，而鸟苷酸环化酶抑制剂亚甲蓝（ＭＢ）增加睡眠、减少觉醒，并可阻断ＳＮＰ的促睡眠效应。结论杏仁核参与睡眠 觉醒调节，杏仁核中ＮＯ具有促进Ｗ、抑制ＳＷＳ效应，这一作用是通过激活鸟苷酸环化酶使ｃＧＭＰ增多实现的。

Effects of nitric oxide in nucleus amygdala on sleep and wakefulness in rats

AIM To investigate the effects of nitric oxide (NO) in nucleus amygdala on sleep and wakefulness (W). METHODS Polysomnography (PSG) and nucleus amygdala microinjection. RESULTS nitric oxide synthase (NOS) inhibitor N nitro L arginine ( L NNA) increased slow wave sleep (SWS) and decreased W, but sodium nitroferricyanide (SNP), a NO donor, not only decreased W but also abolished the sleeppromoting effect of L NNA. L arginine ( L Arg), a precusor of NO, had no direct effect on sleep and W, but blocked the effect of L NNA. Cyclic guanosine monophosphate (cGMP) decreased SWS and increased W, but methylene blue (MB), a guanosine inhibitor, increased SWS and decreased W, and antagonized the W promoting effect of SNP. No significant change of paradoxical sleep (PS) was found in any group. CONCULUSION Nucleus amygdala play an important role in regulating sleep and wakefulness, and NO in nucleus amygdala has W promoting and SWS inhibiting effects mediated by activation of guanylate cyclase and increase of cGMP concentration.