山莨菪碱对红细胞自氧化的影响

目的　研究山莨菪碱（Ａｎｉ）在红细胞自氧化损伤中的作用。方法　在红细胞悬液中加入不同浓度Ａｎｉ，温育２４ｈ，分别用比色法、硫代巴比妥酸荧光法、荧光偏振法测定红细胞溶血度、红细胞膜脂质过氧化物（ＬＰＯ） 、红细胞膜微粘度。结果　Ａｎｉ１－０，１－５ ｍ ｍｏｌ·Ｌ－１ 可显著抑制红细胞自氧化溶血（ 溶血度分别为：０－５８５ ±０－０２８、０－４３９ ±０－０２４，对照组为０－７９８ ±０－０３５ ，Ｐ＜０－０１）、显著降低红细胞膜ＬＰＯ〔分别为：（０－３５９ ±０－０１７） 、（０－３２３ ±０－０１９）μｍｏｌ·ｇ－１ Ｐｒｏ，对照组为（０－４１８±０－０１５）μｍｏｌ·ｇ－１ Ｐｒｏ， Ｐ＜ ０－０１〕、显著降低红细胞膜微粘度〔分别为：（０－３２８±０－０１７）、（０－２９６ ±０－０１９）Ｐａ·ｓ，对照组为（０－３７４ ±０－０１４） Ｐａ·ｓ〕。结论　Ａｎｉ 抗氧自由基引发的膜脂质过氧化、保护细胞膜，对红细胞自氧化损伤有保护作用

Effect of anisodamine on auto oxidation of erythrocytes

AIM To study the effect of anisodamine (Ani) on auto oxidative action of erythrocyte. METHODS Different concentrations of Ani were added to erythrocytes suspension, and suspension was incubated at 37℃ for 24 h in vitro , then erythrocyte hemolysis degree, erythrocyte membrane lipid peroxide (LPO) and erythrocyte membrane microviscosity (EMMV) of suspension were determined with method of colorimetry, thiobarbiturie acid fluorescence, and fluorescence polarization, respectivly. RESULTS Ani could significantly inhibit auto oxidative hemolysis of erythrocytes hemolysis degrees were 0 585±0 028,0 439±0 024 when Ani were 1 0 and 1 5 mmol·L -1 ,respectively, but it was 0 798±0 035 in control group ( P <0 01), LPO of erythrocyte membrane was decreased significantly, it was (0 359±0 017),(0 323±0 019) μmol·g -1 protein,respectively, control group was (0 418±0 015) μmol·g -1 protein ( P <0 01), EMMV was reduced significantly (0 328±0 017) Pa·s and (0 296±0 019) Pa·s, respctively, control group was (0 374±0 014) Pa·s. CONCLUSION Ani can resist membrane lipid peroxidation caused by free radical and protect cell membrane and auto oxidation of erythrocyte.