MK-801对大鼠海马长时程增强的作用

目的观察了ＭＫ ８０１对大鼠海马长时程增强的作用。方法采用大鼠海马离体脑片灌流技术。结果强直刺激（１００Ｈｚ，１ｓ）在大鼠海马ＣＡ１区可以诱发ＬＴＰ的作用；在给１０μｍｏｌ·Ｌ－１ＭＫ ８０１过程中，给予强直刺激仍能诱导ＬＴＰ的形成；强直刺激诱导ＬＴＰ后给予ＭＫ ８０１不能抑制ＬＴＰ的形成；在孵育槽中加入１０μｍｏｌ·Ｌ－１ＭＫ ８０１孵育２ｈ后给予强直刺激不能诱导ＬＴＰ的形成。结论预先给予ＭＫ ８０１可以阻断强直刺激诱导ＬＴＰ的形成。

Effect of MK 801 on long term potentiation in hippocampal CA1 area of rat *

AIM The effect of MK 801 on long term potentiation (LTP) in hippocampal CA1 area was observed. MOTHEDS Electrical stimulation was delivered to the Schaffer commissural fiber system and extracellular recording was performed at the pyramidal cell body layer in CA1 area of hippocampal slices. Population spikes were recorded to study the induction of LTP. RESULT A tetanic stimulation (100 Hz, 1 s) could induce LTP in hippocampal CA1 area of rat. LTP could still be induced when MK 801 was added. LTP could not be inhibited adding MK 801 after the induction of LTP. LTP could not be induced after brain slices were incubated with MK 801 for 2 hours. CONCLUSION Our study suggested that pretreating with MK 801 could inhibit LTP.