| 荧光光谱法测定小鼠血浆中异丙酚的浓度 |
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| 引用本文： | 王惠霞,徐礼鲜,马加海,张惠,周军,李晓晔. 荧光光谱法测定小鼠血浆中异丙酚的浓度[J]. 安徽医药, 2005, 9(8): 586-587 |
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| 作者姓名： | 王惠霞 徐礼鲜 马加海 张惠 周军 李晓晔 |
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| 作者单位： | 第四军医大学口腔医学院麻醉科,陕西,西安,710032;济南军区总医院麻醉科,山东,济南,710032;第四军医大学口腔医学院麻醉科,陕西,西安,710032;第四军医大学药学系化学教研室,陕西,西安,710032 |
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| 摘 要： | 目的应用荧光光谱法测定小鼠血浆中异丙酚的浓度.方法在含不同量异丙酚的小鼠血浆中加入磷酸缓冲液及环己烷/正丁醇,振荡、离心后取上清液测荧光强度(发射波长299 nm,激发波长270 nm).结果异丙酚投入量(0.1～10 mg·L-1)与荧光强度(INT)间有良好的线性关系(r=0.999 6).结论该方法灵敏度高,线性范围大,操作简便,专一性好,可用于临床异丙酚血药浓度的监测.
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| 关 键 词： | 异丙酚 药代动力学 荧光光谱法 小鼠血浆 |
| 收稿时间： | 2005-04-12 |
| 修稿时间： | 2005-04-12 |
Blood concentration determination of propofol in mice with spectrofluorometry |
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| WANG Hui-xia,XU Li-xian,MA Jia-hai,et al. Blood concentration determination of propofol in mice with spectrofluorometry[J]. Anhui Medical and Pharmaceutical Journal, 2005, 9(8): 586-587 |
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| Authors: | WANG Hui-xia XU Li-xian MA Jia-hai et al |
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| Abstract: | AimTo determine the concentration of propofol in blood plasma of mice with spectrofluorometry.MethodsBuffer solution of phosphoric acid and cyclohexane-tertbutanol were added to blood plasma with different concentration of propofol, which were vibrated and centrifugalized. After that, the upper-clear solution was taken to determine the fluorescent intensity (excitation wave 270 nm, emission wave 299 nm).ResultsThe experimental data exhibited a good linear relationship between the logarithms of the fluorescent intensity and the amount of propofd added to the blank plasma in the scale of 0.1~10 mgL~(-1)(r=0.999 6).ConclusionThis method is good at sensitivity. Linearity range is simple.The convenient, economical ,accurate and specific procedure can be applied to determine the concentration of propofol in blood clinically. |
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| Keywords: | propofol pharmacyokinetics spectrofluorometry blood plasma |
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