以表皮细胞及脱细胞真皮构建组织工程皮肤

目的 体外扩增培养表皮细胞，复合脱细胞真皮构建组织工程皮肤，以促进其进一步临床应用。方法 将人表皮细胞进行体外培养扩增后，以生长良好的表皮细胞接种于制备好的异种（猪）无细胞真皮支架上，行气液界面联合体外培养，构建组织工程皮肤。观察细胞生长情况及复合皮结构。结果 培养的表皮细胞扩增良好，脱细胞真皮支架去细胞完全，表皮细胞在脱细胞真皮上可以生长，可体外构建复合皮肤。结论 利用体外扩增的表皮细胞及制备的脱细胞真皮可体外联合构建具有复层结构的组织工程皮肤。

Construction of Tissue-Engineered Skin with Epidermal Cells and Acellular Dermal Matrix

Objective :To culture and proliferate keratinocytes in vitro,and to construct tissue-engineered skin with the cultured cells as well as acellular dermal matrix for tissue-engineered skin.Methods Human keratinocytes were isolated from child forskin dersertded after circumcision procedure,the cultured cells in great shape were seeded onto the surface of acellular dermal matrix.When it was cultured absolutely inside liquid medium for about a week,the upside surface then was lifted outside exposed to air.In this way,tissue-engineered skin was expected to be constructed in vitro.The cultured cells and construction of tissue-engineered skin were observed.Results The cultured cells were observed growing and proliferating well in vitro.With the cells cultured on the surface of acellular dermal matrix with cell-free,tissue-engineered skin could be construted in vitro.Conclusion Constuction of tissue-engineered skin with human keratinocytes as well as acellular dermal matrix in vitro is feasible.