类风湿关节炎关节液来源的间充质干细胞分离鉴定及其对破骨细胞发育的影响

为了研究炎性微环境对于间充质干细胞生物学特性的影响，从类风湿性关节炎病人关节液中分离得到间充质干细胞，以流式细胞术测定其免疫学表型，以成骨和成脂肪诱导检测其多向分化能力，将其与CD14^＋的单核细胞共同培养并以抗酒石酸的酸性磷酸酶染色检测对破骨细胞的调控作用。结果表明：类风湿性关节炎病人关节液中分离得到的间充质干细胞高达CD105，CD73，CD29，CD44，CD166和HLA—ABC，低表达CD34，CD45，CD31，HLA—DR，CD80和CD86；与健康人骨髓间充质干细胞相比，其向成骨细胞和脂肪细胞分化的能力下降，而且其支持更多的破骨细胞生成（P〈0．05）。结论：类风湿性关节炎病人关节液中存在的间充质干细胞具有与骨髓间充质干细胞相似的表型，但是其多向分化能力下降，更有意义的是其支持破骨细胞生成的能力增强。本研究结果有助于了解病理微环境中的间充质干细胞生物学特性。

Identification of Mesenchymal Stem Cells Derived from Rheumatoid Arthritis Synovial Fluid and Their Regulatory Effect on Osteoblast Formation

This study was purposed to investigate the influence of inflammatory microenvironment on mesenchymal stem cells （MSCs） and regulatory effect of MSCs on osteoblast formation. The MSCs were isolated from synovial fluid of patients with rhemmatoid arthritis （RASF-MSCs） and were cultured, the immunotypes of RASF-MSCs were detected by flow cytometry, the ability to differentiate RASF-MSCs into obseoblasts and adipocytes was determined by means of osteogenic and adipogenic induction, the regulatory effect of RASF-MSCs on osteoblast formation was assayed by co- culturing RASF-MSCs whth CD14 ^＋ monocytes and in situ tartrate-resistant acid phosphatase staining. The results showed that RASF-MSCs highly expressed CD105, CD73, CD29, CD44, CD166 and HLA-ABC. Meanwhile, they lowly expressed CD34, CD45, CD31, HLA-DR, CD80 and CD86. However, RASF-MSCs decreased multi-differentiation capability as compared with BM-MSCs. More interestingly, RASF-MSC significantly promoted osteoclasts formation （p 〈0.05） when co-cultured with monocytes. It is concluded that MSCs from rheumatoid arthritis synovial fluid exert typical MSC phenotypes but displayed decline of multi-differentiation capability. RASF-MSCs especially show promoting effect on osteoclastogenesis. The findings of this study may contribute to the understanding biological behavior of MSCs in pathological microenvironment.