米非司酮对子宫内膜异位症大鼠survivin表达的影响

目的:探讨米非司酮对子宫内膜异位症(endometriosis,EMS)大鼠survivin表达的影响。方法:子宫内膜异位症大鼠随机分为正常对照组、低剂量和高剂量米非司酮组,灌胃4周后,分别取子宫内膜组织用免疫组化法测survivin的表达。结果:(1)各组在位内膜survivin的表达强度,高剂量组低于低剂量组(P<0.05)和正常对照组(P<0.05),而低剂量组和正常对照组间差异无显著性。(2)各组间异位内膜survivin的表达强度均有明显差异,高剂量组低于低剂量组(P<0.05)和正常对照组(P<0.01),低剂量组也明显低于正常对照组(P<0.01)。(3)不同组内在位、异位内膜survivin的表达强度,高剂量、低剂量组异位内膜均明显低于在位内膜(P<0.01),而正常对照组异位内膜则高于在位内膜(P<0.05)。结论:米非司酮治疗EMS可能与降低survivin的表达有关。

Effect of mifepristone on-expression of survivin in endometriotic rats

Objective To explore the effect of mifepristone on expression of survivin in endometriotic rats. Methods Endometriotic rats were randomly divided into control group and mifepristone group (including low-dose and high -dose groups). 4 weeks after gastrogavage with tuwen-80 only (control group) or tuwen-80 plus mifepristone 0.62 mg/d (low -dose group) or 1.24 mg/d(high-dose group), all of the rats were killed and tissues of ectopic and eutopic endometrium were removed for detecting survivin-expression via immunohistochemistry (SP method).Results (1) In the eutopic endometium,the expression intensity of survivin in the high-dose group was lower than that in the low-dose group and the control group (P< 0.05). (2) In the ectopic endometium , the expression intensity of survivin in the high -dose group was lower than that in the low -dose group (P< 0.05) and the control group (P< 0.01). The expression intensity of survivin in the low dose group was lower than that in the control group (P< 0.01).(3) In both high -dose and low -dose groups, the expression intensity of survivin in the ectopic endometrium was lower than that in the eutopic endometrium (P< 0.01), but in the control group, the expression intensity of survivin in the ectopic endometrium was higher than that in the eutopic endometrium (P< 0.05). Conclusion The treatment effect of mifepristone on EMS might be related with decreased expression of survivin.