A functional assay of protein C in human plasma |
| |
| Authors: | C M Hickton P Felding K Ikeda G Martinsson I M Nilsson |
| |
| Institution: | 1. Department of Medicine, Nephrology Division, Icahn School of Medicine at Mount Sinai, New York, New York, USA;2. Renal Program, James J. Peters Veterans Affairs Medical Center, Bronx, New York, USA;1. Department of Food Technology, Federal University of Technology - Paraná, Francisco Beltrão, PR, Brazil;2. School of Chemistry and Food, Federal University of Rio Grande, Rio Grande, RS, Brazil;3. Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil;4. Federal Rural University of Amazonia, Capitão Poço, PA, Brazil;5. Department of Biotechnology, State University of Maringá, Maringá, PR, Brazil;1. Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, 11 Mandalay Road, 308232, Singapore |
| |
| Abstract: | A functional assay for protein C in plasma is described in which barium eluates of plasma are incubated with bovine thrombin and rabbit thrombomodulin to activate protein C. The activated protein C solution is added to an activated partial thromboplastin time (APTT) system containing normal plasma and an APTT reagent (Dade ActinR). The prolongation of coagulation time after recalcification in this system is taken as a measure of the anticoagulant activity of protein C. When expressed as per cent of the value in pooled normal plasma, the results obtained by this method in 34 normal controls and in 3 untreated patients with protein C deficiency were very similar to those obtained by radioimmunoassay of protein C. In 2 patients with protein C deficiency and 23 patients without, all on dicoumarol or warfarin treatment, the anticoagulant activity of protein C was less than its antigen concentration. The day to day analytical coefficient of variation (SD/mean) was 12% at the 100% level (n = 12), and 10% at the 25% level (n = 12). |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
