盐酸利多卡因体外对精子作用的扫描电镜观察

目的探讨盐酸利多卡因（LHA）体外对人精子形态结构改变的机制。方法选择健康生育男性8名,年龄23～32岁,禁欲3～7d后,手淫取精。分为对照组（A组）,实验B组（LHA终浓度为5mg/mL）、实验C组（LHA终浓度为15mg/mL）。体外孵育1、2h后,采用S3400N扫描电镜观察各组精子形态的改变。结果与A组相比,实验B组1h后精子形态结构改变差异无统计学意义（P〉0.05）,2h后差异有统计学意义（P〈0.05）;实验C组1h后差异有统计学意义（P〈0.05）,2h后差异更为明显（P〈0.01）。结论随着LHA终浓度的增加和孵育时间的延长,LHA对精子形态结构改变越来越明显。

Scanning electronic microscope observation of the action of lidocaine hydrochloric acid to human sperm in vitro

Objective To investigate the mechanism of the change of sperm shape after using lidocaine hydrochloric acid （ LHA） in vitro. Methods 8 healthy males,whose age was 23-32 years old,and abstinence 3-7 d,were enrolled in the study. The sperm was collected through the masturbation. The specimens were classified into control group A and experiment group B,C. The LHA were added to the experiment groups respectively at the different concentrations. The concentrations were 5 mg/mL and 15 mg/mL respectively. After fostering 1 h and 2 h in vitro,the changes of sperm shape were examined in the group A ,B and C through the scanning electronic microscope S3400N. Results Compared with group A,group B did not show obvious difference after fostering 1 h（ P〈0. 05） ,but the difference was remarkable after fostering 2 h（ P〈0. 05）. Group C showed difference after fostering 1 h（ P〈0. 05） ,and more remarkable difference after fostering 2 h （ P〈0. 01）. Conclusions With increasing end concentration and extended time,the influence of LHA on the change of sperm shape is more obvious.