Human Monoclonal Antibodies against Cytokeratin 18 Generated from Patients with Gastric Cancer |
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| Authors: | Tsutomu Abe Masayuki Fukumoto Keiko Tsuchiya Kentaro Kuramochi Tadaaki Furuta Shinji Togoh Kiyoshi Nishiyama Shuji Tsuchiya |
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| Affiliation: | Medical Science Laboratory, Asahi Chemical Industry Co., Ltd., 2-1 Samejima, Fuji, Shizuoka 416;Second Department of Surgery, School of Medicine, Yokohama City University, 3-4 Urafune-cho, Minami-ku, Yokohama 232 |
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| Abstract: | By co-culturing regional lymph node B-cells and HAT-sensitive mutant cells obtained from RPMI-1788 cells, no less than 20,000 Epstein-Barr (EB)-transformed colonies were obtained from 32 patients with gastric cancer. From B-cell cultures generating antibodies reactive with gastric cancer tissues as well as cultured gastric cancer cells, two EB-transformed cell clones termed C418–59 and C1218–39 were isolated. Both of them produced human IgM-class antibodies, termed Mab418–59 and Mab 1218–39, respectively. Both antibodies reacted with an antigen with a molecular weight of 45 kd existing in gastric cancer MKN-45, MKN-1, and Kato-III cells, and also with all of 4 adenocarcinomas of the stomach in paraffin sections. The antigen recognized by both antibodies was identified as a kind of cytoskeletal protein, cytokeratin 18, In this study, it was confirmed that B-cell clones generating autoantibodies against cytokeratin 18 were present in some patients with gastric cancer. |
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| Keywords: | Human monoclonal antibody Cytokeratin 18 Tissue polypeptide antigen |
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