| 按蚊TEP1基因的原核表达及鉴定 |
| |
| 引用本文： | 张健,黄复生,宋鹏,段建华,徐文岳,邱宗文. 按蚊TEP1基因的原核表达及鉴定[J]. 中国人兽共患病杂志, 2007, 23(12): 1184-1186 |
| |
| 作者姓名： | 张健 黄复生 宋鹏 段建华 徐文岳 邱宗文 |
| |
| 作者单位： | 第三军医大学基础部病原生物学教研室,第三军医大学基础部病原生物学教研室,第三军医大学基础部病原生物学教研室,第三军医大学基础部病原生物学教研室,第三军医大学基础部病原生物学教研室,第三军医大学基础部病原生物学教研室 重庆400038,重庆400038,重庆400038,重庆400038,重庆400038,重庆400038 |
| |
| 摘 要： | 目的原核表达斯氏按蚊TEP1蛋白。方法PCR方法从斯氏按蚊扩增TEP1基因,并插入原核表达载体pQE-80L,构建重组表达质粒pQE-80L/TEP1。转化DH5α菌,IPTG诱导表达融合蛋白及质谱鉴定。结果成功表达重组质粒,经过质谱鉴定为斯氏按蚊TEP1分子。结论成功表达了按蚊TEP1蛋白为后续的抗体制备及TEP1功能研究奠定了基础。
|
| 关 键 词： | 按蚊 含硫脂蛋白 原核表达 质谱 |
| 文章编号： | 1002-2694(2007)12-1184-03 |
| 收稿时间： | 2007-12-20 |
| 修稿时间： | 2007-06-11 |
Prokaryotic expression and identification of anophele TEP1 |
| |
| ZHANG Jian,HUANG Fu-sheng,SONG Peng,DUAN Jian-hua,XU Wen-yue,QIU Zhong-wen. Prokaryotic expression and identification of anophele TEP1[J]. Chinese Journal of Zoonoses, 2007, 23(12): 1184-1186 |
| |
| Authors: | ZHANG Jian HUANG Fu-sheng SONG Peng DUAN Jian-hua XU Wen-yue QIU Zhong-wen |
| |
| Abstract: | To obtain the Anopheles stephensi(As) TEP1,its gene was amplified by PCR from the Anopheles stephensi.and the fragment was inserted into vector pQE-80L to construct recombinant plasmid pQE-80L/TEP1.Following transformation into E.coli DH5α,the fusion protein was expressed under IPTG induction.SDS-PAGE and mass spectrometry(MS) were used to determine the expression of the expected protein.The fusion p rotein was successfully expressed and the TEP1 protein was confirmed by MS.The expression of the recombinant TEP1 protein has laid a foundation for further antibody preparation and function studies of TEP1 protein. |
| |
| Keywords: | nophele TEP1 prokaryotic expression mass spectrometry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国人兽共患病杂志》浏览原始摘要信息 |
|
点击此处可从《中国人兽共患病杂志》下载全文 |
|
