过度运动对大鼠肱二头肌长头肌腱损伤和腱细胞凋亡的影响

目的探讨过度运动对大鼠肱二头肌长头肌腱组织和腱细胞凋亡的影响。方法采用随机分组法,将40只Sprague-Dawley(SD)大鼠随机均分为8组,1组对照组,7组为实验组,对照组笼中自由活动,实验组以17m/min下坡(10°)跑步,每天1h、每周5d建立过度运动模型。对照组0w取材,实验组中的第1～6组分别于运动1、2、3、4、5、6w后取材,第7组运动6w后休息2w即第8w取材。苏木素伊红染色(HE染色),观察组织学变化,通过TUNEL法和Caspase-3(天冬氨酸特异性半胱氨酸蛋白酶-3)活性检测法检测腱细胞凋亡。结果 (1)过度运动的肱二头肌长头肌腱与对照组比较,1、2、3和4w肱二头肌长头肌腱胶原纤维排列逐渐出现排列不规则,4w时较为显著,肌腱纤维出现明显排列紊乱,部分发生变性及着色变浅,纤维细胞数量增加;与对照组相比,5、6、8w未发生显著变化。(2)TUNEL法和Caspase-3活性检测两种方法的结果基本一致,与对照组相比,1、2w肱二头肌长头肌腱腱细胞凋亡数无明显差异(P>0.05);TUNEL染色显示3、4w腱细胞凋亡数明显增加(P<0.05),Caspase-3活性检测显示4w腱细胞凋亡数明显增加(P<0.05);5、6w腱细胞凋亡数下降,与对照组无明显差异(P>0.05);8w腱细胞凋亡数基本回到0w对照组水平。结论过度运动可引起大鼠肱二头肌长头肌腱组织退变和腱细胞凋亡。

Study on rat long head of biceps tendon injury and apoptosis of tenocyte by Overuse

Objective To investigate the influence of overuse for the rat long head of biceps tendon and the apoptosis of tenocyte.Methods 40 Sprague-Dawley rats was randomly divided into eight groups,one group as the control group,seven groups as the experimental groups.Adult rats were subjected to an overuse protocol that consists of downhill running(10% grade) at 17 m/min for 1 h/day,5 days/week.The tendons of control group were harvested at 0 w,the tendons of experimental groups were harvested at 1,2,3,4,5,6,8 w.Th...