HA230树脂灌流吸附后血中灭多威的清除效率

目的研究血中灭多威的分析方法及血样灌流吸附后对毒物的定量清除率。方法采用HA230树脂灌流装置对中毒血样进行灌流吸附,将吸附后血样经乙酸乙酯萃取后,用带有紫外检测器的高效液相色谱法测定其含量并计算清除率。结果分析方法的检出限为0.02 ng,低、中、高3种浓度的加标回收率为93.32%～103.24%,相对标准偏差为2.63%～4.88%。灌流吸附后血中毒物的清除效率为98.68%～99.64%。结论用HA230树脂吸附剂进行血液的灌流吸附,能清除大部分毒物,迅速降低血中灭多威的浓度,对抢救重度灭多威中毒的危重病人有重要价值。

HPLC analysis of methomyl in blood and quantitative cleanup-rate to poison by hemoperfusion

Objective To study the cleanup-rate of methomyl in blood sample after hemoperfusion.Methods We put methomyl standard solution into the health human blood,and took it through a HA230 resin hemoperfusion cartridge having the huge absorption capacity.Then the blood samples were extracted by acetic ether,at last the contents of methomyl were determined by high performance liquid chromatography（HPLC）.Results The limit line of methomyl in mankind blood was 0.02ng.The recoveries of low,middle and high three kinds added were between 93.32% and 103.24%.The relative standard deviations（RSD） was between 2.63% and 4.88%.The cleanup-rate was 98.68%~99.64%.Conclusion The poisons mostly are removed out from blood after poisons absorption through HA230 resin hemoperfusion and the concentration of methomyl in blood is reduced rapidly.